This experiment was conducted to investigate the effects of Qizao extract on the laying performance,egg quality,reproductive hormones and follicle development of Yugan black bone chickens.A total of 120 Yugan black-bone chickens with good body condition at 240 days of age were randomly divided into 4 groups,with 6 replicates in each group and 5 chickens in each replicate.The control group was fed a basal diet.The experimental group added 100,200,400 mg/kg of Qizao extract.The adaptation period lasted for 56 days.The results showed that:(1)The Qizao extract had no effect on the egg production rate,feed-to-egg ratio,average egg weight and average daily feed intake of Yugan black-bone chickens(P>0.05).(2)The yellowness of egg shells of Yugan black bone increased linearly with the increase of Qizao extract(P<0.05),and the Haugh unit,egg yolk index and eggshell redness showed a quadratic curve(P<0.05).(3)On the 28th day of the experiment,the E2 content in the serum of Yugan black-bone chickens showed a quadratic curve with the increase of the concentration of Qizao extract(P<0.05),and the FSH and LH contents increased linearly with the increase of the concentration of Qizao extract(P<0.05);On the 56th day of the experiment,the extract of Qizao extract had no significant effect on the serum E2,FSH and LH content of Yugan black-bone chickens(P>0.05).(4)ESRβ and GnRH1 in the ovary of Yugan black-bone chickens increased linearly with the increase of Qizao extract(P<0.01),and FSHR showed a quadratic curve with the increase of Qizao extracts(P<0.05).It was concluded that the addition of Qizao extract to the diet had no significant effect on the performance of Yugan black-bone chicken,but could improve egg quality,reproductive hormone content and receptor expression.Under the conditions of this experiment,the suitable addition amount was 200 mg/kg.
Yugan black-bone chickensproduction performanceegg qualityreproductive hormonesfollicle development