黄芩根提取物促进大鼠脂质代谢机制研究
Effects of Scutellaria L.on PPARγ,APN and AMPK signaling pathways in the kidneys of obese hypertensive rats
张前熙 1王超 1严鸿林 2肖熠3
作者信息
- 1. 四川卫生康复职业学院,四川自贡 643000
- 2. 西南科技大学,四川绵阳 621010
- 3. 四川农业大学动物营养研究所,四川成都 611130
- 折叠
摘要
文章旨在探讨黄芩根提取物对大鼠脂肪沉积的影响及其作用机制.试验采用 8 周龄雄性SD大鼠 24 只,随机分为普通日粮组和高脂日粮组,每组再随机分为对照组和黄芩处理组,即对照组(CTL)、普通处理组(TRT)、高脂组(F-CTL)、高脂处理组(F-TRT),每组 15 只SD大鼠.TRT与F-TRT每天用黄芩根提取物制剂灌胃,CTL和F-CTL给予等量蒸馏水灌胃,处理 10 周后对比分析各组体重、体脂率、血糖、血液脂肪指标、APN、PPARγ、AMPK水平及其mRNA表达水平.结果表明,对于正常的健康大鼠,黄芩根提取物处理对其体重和脂质代谢并不会产生显著影响.而在肥胖状态下,前者可通过提升脂肪组织中脂联素水平,激活PPARγ/AMPK信号通路,进而促进脂质分解代谢,从而降低大鼠体重、体脂率和血压,并改善由高脂日粮引发的血糖升高.结论,黄芩根提取物可通过激活脂肪组织中APN/PPARγ/AMPK信号通路发挥抑制肥胖与降压的双重作用.
Abstract
This study was conducted to investigate the effect of Scutellaria L.root extract on fat deposition in rats and its mechanism.Twenty-four 8-week-old male SD rats were randomly divided into normal diet group and high-fat diet group,and each group was randomly divided into control group and Scutellaria L.root extract treatment group,namely control group(CTL),normal treatment group(TRT),high-fat group(F-CTL),high-fat treatment group(F-TRT),15 SD rats in each group.The TRT and f-trt groups were intragastrically administered with Scutellaria baicalensis root extract preparation every day,and CTL and f-ctl were intragastrically administered with the same amount of distilled water.After 10 weeks of treatment,the body weight,body fat rate,blood glucose,blood fat index,APN and PPAR of each group were compared and analyzed PPARγ,AMPK level and their mRNA expression level.The results showed that,for normal healthy rats,the treatment of Scutellaria L.root extract had no significant effect on body weight and lipid metabolism.However,in obesity,the former can activate PPARγ/AMPK signaling pathway by increasing adiponectin levels in adipose tissue and further promotes lipid catabolism,thereby reducing body weight,body fat rate and blood pressure in rats,and improving the increase of blood glucose caused by high-fat diet.Conclusion:Scutellaria L.root extract can activate APN/PPARγ/AMPK signaling pathway in adipose tissue,and plays a dual role in inhibiting obesity and lowering blood pressure.
关键词
黄芩根提取物/肥胖/高血压/APN/PPARγ/AMPKKey words
scutellaria L.root extract/obesity/high blood pressure/APN/PPARγ/AMPK引用本文复制引用
基金项目
四川省科技厅项目(2021YFH0157)
出版年
2024
NETL
NSTL
维普
万方数据