To improve the nutritional value of soybean meal and realize the high-value utilization of soybean meal,this experiment was based on the solid-state method,in which soybean meal was degraded by acid protease in a constant temperature box at 50 ℃.Taking the productivity of amino acid nitrogen as an index,the optimal technological conditions of soybean meal enzymolysis were determined by controlling variables.The experimental results showed that when the amount of acid protease was 0.5％of the weight of soybean meal dry powder,the water content was 33.3％and the enzymolysis time was 72 h.The highest production rate of amino acid nitrogen could reach 0.060 g N/g soybean meal,and the significance analysis of the experimental data showed that there was a significant difference between the enzyme supplemental level and the amino acid nitrogen production rate(P＜0.05).There were 20 kinds of amino acids produced by enzymatic hydrolysis of soybean meal,among which tryptophan,alanine,and isoleucine account for 42.36％,essential amino acids for feeding account for 68.27％,and amino acids for regulating intestinal function account for 20.22％of the total amino acids.This experiment was of great significance to improve the nutritional value of soybean meal,realize the efficient development and utilization of soybean meal,reduce the production cost,and reduce the dependence on animal protein-derived feed.
维普
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