Screening of high-yielding benzoilactic acid strains and optimization of fermentation conditions
Phenyl lactic acid can both extend the shelf life of feed and improve the performance of livestock and poultry.However,the current yield of bioindustrial production of phenyl lactic acid is very low.To find a high-yielding strain,a high-yielding phenyl lactic acid strain was screened from kimchi and identified as Lactobacillus plantarum by 16S rDNA sequencing.The fermentation conditions were optimized by single-factor test and orthogonal test,and the optimal solution between exogenous additive and temperature was determined by response surface test to obtain the high yield of phenyl lactic acid.The results showed that the orthogonal and response surface tests determined that the expression of phenyllactic acid reached 374.26 mg/L with 25 g/L of glucose addition,5 g/L of corn pulp addition,100 μL of inoculum,72 h of fermentation time,8 g/L of phenylalanine addition,5 g/L of citric acid addition,and 33 ℃ of reaction temperature,which was better than that of 38.97 mg/L before optimization.The optimized Lactobacillus plantarum could produce high benzolactic acid and solve the problem of low expression of the original strain.
万方数据
维普
