In this study,a strain named HHL with β-glucosidase production was screened from mycelium bank of the laboratory,and was identified as Aspergillus oryzae by ITS sequence analysis.The enzymatic activity of β-glucosidase as response value,the fermentation conditions were optimized by single factor and orthogonal tests.The results showed that the optimum conditions of β-glucosidase activity of HHL were as follows:the modified Czapek's medium was used as the fermentation medium,fermentation time was 144 h,fermentation temperature was 35 ℃,inoculums size was 8％,pH was 6.0.Under the optimal conditions,the β-glucosidase activity was 48.74 U/mL.
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