In order to explore the effects of different doses of CH on broiler production performance,intestinal antioxidant capacity and intestinal barrier function,a total of 240 1-day-old broiler were randomly assigned to 4 groups with 4 replicates per group and 15 birds per replicate.Broilers were fed a basal diet(control group),the basal diet supplemented with 1,3,5 g/kg CH from 1～28 days of ages.The results showed as follows:(1)The average daily weight gain and average feed intake in the CH-3 group increased by 7.2％and 11.9％compared with the CON group(P＜0.05).(2)The broiler bursal index and the content of serum immunoglobulin M(IgM)in the CH-1 group increased by 48.8％and 16.6％compared with the CON group(P＜0.05);the content of serum immunoglobulin G(IgG)in the CH-1 and CH-3 group increased by 11.2％and 12.4％compared with the CON group(P＜0.05).(3)The content of serum malondialdehyde(MDA)in the CH-1 group was reduced by 22.4％(P＜0.05)compared with the CON group;the levels of serum superoxide dismutase(SOD)in the CH-1 group increased by 12.7％(P＜0.05).(4)The ileum villus height and the ratio of ileum villus height to crypt depth in the CH-1 group increased by 19.1％and 18.6％compared with the CON group(P＜0.05);(5)The gene expression levels of GLUT-2,GSH-Px,ZO-1,Occludin-1,IL-4 and IL-10 in ileum mucosa in CH-1 group increased by 41.0％,32.4％,39.6％,38.5％,23.8％and 38.0％compared with the CON group(P＜0.05);the gene expression levels of Claudin in ileum mucosa in CH-3 group increased by 62.4％compared with the CON group,the gene expression levels of MyD88 in ileum mucosa in the CH-3 group decreased by 14.5％compared with the CON group,the gene expression levels of superoxide dismutase(SOD 1)and cationic amino acid transporter protein(CAT)in ileum mucosa in the CH-5 group increased by 39.4％and 48.5％compared with the CON group(P＜0.05);The expression of Nrf2 in ileum mucosa increased by 39.4％,30.3％,respectively,in all dose group compared with the CON group(P＜0.05).In conclusion,under the condition of this experiment,supplementation of 1 mg/kg CH could significantly improve growth performance,enhance the status of antioxidant function and immune,promote intestinal villi development,improve intestinal integrity.
Callicarpa nudiflora HookHainan Wenchang broilergrowth performanceantioxidant functionimmune function
维普
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