摘要
为探究不同剂量裸花紫珠粉(Callicarpa nudiflora Hook,CH)对肉鸡生长性能、肠道抗氧化能力和肠道屏障功能的影响,试验选取1日龄健康海南文昌鸡240只,随机分为4个处理组,每个处理组4个重复,每个重复15只鸡.空白组(CON组)饲喂基础饲粮,试验组分别在基础饲粮中添加裸花紫珠粉1 g/kg(CH-1组)、3 g/kg(CH-3组)和5 mg/kg(CH-5组),自由采食饮水,试验期28 d.结果显示:(1)CH-3组的日采食量和日增重较CON组增加了 7.2％和11.9％(P＜0.05).(2)CH-1组肉鸡法氏囊指数和血清免疫球蛋白M(IgM)含量较CON组提高了 48.8％和16.6％(P＜0.05);CH-1组和CH-3组肉鸡血清免疫球蛋白G(IgG)含量较CON组提高了 11.2％和12.4％(P＜0.05).(3)CH-1组肉鸡丙二醛(MDA)含量较CON组降低了 22.4％(P＜0.05);CH-1组肉鸡血清超氧化物歧化酶(SOD)活性提高了 12.7％(P＜0.05).(4)CH-1组肉鸡回肠的绒毛高度(VH)和绒隐比(V/C)较CON组提高了19.1％和18.6％(P＜0.05).(5)CH-1组肉鸡回肠葡萄糖转运蛋白-2(GLUT-2)、谷胱甘肽过氧化物酶(GSH-Px)、带状闭合蛋白-1(ZO-1)、咬合蛋白(Occludin)、白介素-4(IL-4)和白介素-10(IL-10)基因表达量较CON组提高了41.0％、32.4％、39.6％、38.5％、23.8％和38.0％(P＜0.05);CH-3组肉鸡回肠屏障基因闭合蛋白(Claudin)基因表达量较CON组提高了 62.4％,CH-3组肉鸡回肠髓样分化因子基因(MyD88)基因表达量较CON组降低了 14.5％,CH-5组肉鸡回肠超氧化物歧化酶-1(SOD1)和阳离子氨基酸转运蛋白(CAT)基因表达量较CON组提高了 39.4％和48.5％(P＜0.05);各剂量组肉鸡回肠Nrf2基因表达量较CON组分别提高了 39.4％、30.3％、41.3％(P＜0.05).综上所述,日粮添加1 g/kg裸花紫珠粉可提高肉鸡生长性能,增强机体抗氧化功能和免疫力,促进肠道绒毛发育.
Abstract
In order to explore the effects of different doses of CH on broiler production performance,intestinal antioxidant capacity and intestinal barrier function,a total of 240 1-day-old broiler were randomly assigned to 4 groups with 4 replicates per group and 15 birds per replicate.Broilers were fed a basal diet(control group),the basal diet supplemented with 1,3,5 g/kg CH from 1～28 days of ages.The results showed as follows:(1)The average daily weight gain and average feed intake in the CH-3 group increased by 7.2％and 11.9％compared with the CON group(P＜0.05).(2)The broiler bursal index and the content of serum immunoglobulin M(IgM)in the CH-1 group increased by 48.8％and 16.6％compared with the CON group(P＜0.05);the content of serum immunoglobulin G(IgG)in the CH-1 and CH-3 group increased by 11.2％and 12.4％compared with the CON group(P＜0.05).(3)The content of serum malondialdehyde(MDA)in the CH-1 group was reduced by 22.4％(P＜0.05)compared with the CON group;the levels of serum superoxide dismutase(SOD)in the CH-1 group increased by 12.7％(P＜0.05).(4)The ileum villus height and the ratio of ileum villus height to crypt depth in the CH-1 group increased by 19.1％and 18.6％compared with the CON group(P＜0.05);(5)The gene expression levels of GLUT-2,GSH-Px,ZO-1,Occludin-1,IL-4 and IL-10 in ileum mucosa in CH-1 group increased by 41.0％,32.4％,39.6％,38.5％,23.8％and 38.0％compared with the CON group(P＜0.05);the gene expression levels of Claudin in ileum mucosa in CH-3 group increased by 62.4％compared with the CON group,the gene expression levels of MyD88 in ileum mucosa in the CH-3 group decreased by 14.5％compared with the CON group,the gene expression levels of superoxide dismutase(SOD 1)and cationic amino acid transporter protein(CAT)in ileum mucosa in the CH-5 group increased by 39.4％and 48.5％compared with the CON group(P＜0.05);The expression of Nrf2 in ileum mucosa increased by 39.4％,30.3％,respectively,in all dose group compared with the CON group(P＜0.05).In conclusion,under the condition of this experiment,supplementation of 1 mg/kg CH could significantly improve growth performance,enhance the status of antioxidant function and immune,promote intestinal villi development,improve intestinal integrity.
基金项目
海南省重点研发项目(ZDYF2023XDNY067)
十四五国家重点研发项目(2022YFD1801103)
维普
万方数据