Preliminary exploration on the effects and mechanisms of plant polysaccharides on antioxidant and immune indexes of different tissues of juvenile Acipenser dabryanus
To investigate the effects of Astragalus polysaccharides and Poria cocos polysaccharides on the antioxidant and immune abilities of different tissues and organs in juvenile Acipenser dabryanus.In the experiment,90 young Acipenser dabryanus fish were randomly divided into a control group,APS group,and PCP group.Each group had 3 replicates,with 10 experimental fish per replicate.Astragalus polysaccharides and Poria cocos polysaccharides were added to the basic diet according to their body weight,and were fed by gavage.The experimental period was 20 days.At the end of the experiment,15 experimental fish were randomly selected from each experimental group to collect tissues or organs such as blood,liver,kidneys,muscles,intestines,and body surface mucus.The double antibody sandwich method was used to detect antioxidant and immune indicators,and the impact of plant polysaccharides on the antioxidant and immune indicators of different tissues and organs of juvenile Acipenser dabryanus was analyzed.The results showed that:(1)Compared with the control group,Astragalus polysaccharides significantly enhanced the activity of SOD in tissues such as serum,liver,kidney,intestine,and muscle of juvenile Acipenser dabryanus(P＜0.05),with an increase rate of 43.36％,60.27％,43.75％,64.65％,and 56.87％,respectively;It could significantly enhance the activity of CAT in tissues such as serum,liver,intestine,and body surface mucus(P＜0.05),with an increase rate of 27.97％,38.83％,126.43％,and 47.93％,respectively;It could significantly enhance the activity of POD in tissues such as serum,kidneys,and muscles(P＜0.05),with an increase rate of 12.39％,22.80％,and 14.14％,respectively;It could significantly enhance the activity of GSH-Px in tissues such as serum,liver,kidneys,intestines,muscles,and body surface mucus(P＜0.05),with an increase rate of 24.34％,33.96％,34.65％,25.00％,15.73％,and 38.96％,respectively;It could significantly enhance T-AOC in tissues such as serum,liver,kidneys,muscles,and body surface mucus(P＜0.05),with an increase rate of 16.88％,20.36％,10.59％,12.07％,and 13.56％,respectively;It can significantly reduce the content of MDA in tissues such as serum,liver,kidneys,intestines,muscles,and body surface mucus(P＜0.05),with a reduction rate of 29.14％,25.18％,18.15％,28.53％,22.20％,and 24.40％,respectively.Poriacocos polysaccharides could significantly enhance the activity of SOD in the intestines,muscles and other tissues of juvenile Acipenser dabryanus(P＜0.05),with an increase rate of 71.95％and 43.00％,respectively;It could significantly enhance the activity of CAT in tissues such as kidneys and body surface mucus(P＜0.05),with an increase rate of 23.25％and 51.29％,respectively;It could significantly enhance the activity of POD in serum(P＜0.05),with an increase rate of 16.87％;It could significantly enhance the activity of GSH-Px in serum,kidneys,and body surface mucus(P＜0.05),with an increase rate of 21.78％,19.69％,and 36.81％,respectively;It could significantly enhance T-AOC in tissues such as serum,kidneys,intestines,and body surface mucus(P＜0.05),with an increase rate of 11.69％,13.24％,8.23％,and 10.17％,respectively;It could significantly reduce the content of MDA in serum,intestinal and other tissues(P＜0.05),with a reduction rate of 29.86％and 24.03％,respectively.(2)Compared with the control group,Astragalus polysaccharides could significantly enhance the IgG content in the serum,liver and other tissues of juvenile Acipenser dabryanus(P＜0.05),with an increase rate of 34.23％and 39.39％;It could significantly enhance the content of IgM in serum,kidney and other tissues(P＜0.05),with an increase rate of 23.16％and 37.13％;It could significantly enhance the content of C3 and C4 in serum(P＜0.05),with an increase rate of 38.24％and 27.88％,respectively;It could significantly significantly enhance IFN-γ in kidney tissue(P＜0.05),with an increase rate of 41.04％;It could significantly enhance the activity of AKP in serum(P＜0.05),with an increase rate of 25.99％;It could significantly enhance the activity of LSZ in tissues such as serum,liver,kidneys,intestines,and body surface mucus(P＜0.05),with an increase rate of 59.75％,50.00％,75.00％,37.50％,and 66.50％,respectively;It could significantly enhance the content of TP in tissues such as serum,liver,kidneys,intestines,and body surface mucus(P＜0.05),with an increase rate of 39.37％,34.65％,21.24％,55.42％,and 36.38％,respectively.Poriacocos polysaccharides could significantly enhance the IgG content in the serum tissue of juvenile Yangtze sturgeon(P＜0.05),with an increase rate of 26.59％;It could significantly enhance the content of C3 in serum tissue(P＜0.05),with an increase rate of 44.50％;It can significantly enhance the activity of LSZ in tissues such as serum,liver,kidneys,and intestines(P＜0.05),with an increase rate of 36.55％,33.33％,25.00％,and 25.00％,respectively;It can significantly enhance the content of TP in tissues such as intestines and body surface mucus(P＜0.05),with an increase rate of 36.56％and 27.23％,respectively.From this,it could be concluded that both Astragalus polysaccharides and Poriacocos polysaccharides had an improvement effect on the antioxidant and immune functions of different tissues and organs in juvenile Acipenser dabryanus,with Astragalus polysaccharides having a more significant effect.
Acipenser dabryanusAstragalus polysaccharidesPoria cocos polysaccharidesantioxidant indeximmune index
万方数据
维普
