Explore the effects of Clostridium butyricum(CB)on the growth performance,serum biochemical indicators,and immune indicators of weaned piglets.200 healthy hybrid piglets with an initial weight of 8.14±0.07kg and similar age were randomly divided into 4 groups of 5 replicates with 10 replicates each.separately.Add 0,1,5,and 10 to the base diet separately×109 CFU/kg of Clostridium butyricum was used as the experimental diet.The experiment lasted for 35 days.Feed addition 1~10×108 CFU/kg Clostridium butyricum significantly increased the average daily weight gain of piglets in the treatment group by 4.8%,6.65%,and 7.4%,respectively(P<0.05);Feed addition 1~10×108 CFU/kg Clostridium butyricum significantly reduced the average daily feed intake of piglets in the treatment group by 2.9%,5.2%,and 4.6%,respectively(P<0.05);Feed addition 1~10×108 CFU/kg Clostridium butyricum significantly reduced the feed to weight ratio of piglets in the treatment group by 7.4%,11%,and 11.2%,respectively(P<0.05);In addition,the addition of Clostridium butyricum to the diet had no significant effect on the final body weight of piglets(P<0.05).Feed addition 5~10×108 CFU/kg Clostridium butyricum significantly increased the total serum protein content of piglets(P<0.05),and significantly reduced the serum urea nitrogen and triglyceride content of piglets(P<0.05);Feed addition 5×108 CFU/kg Clostridium butyricum significantly increased the serum albumin content of piglets(P<0.05);In addition,Clostridium butyricum had no significant effect on the levels of serum albumin,glucose,and total cholesterol in piglets(P>0.05).Feed addition 5~10×108 CFU/kg Clostridium butyricum significantly increased the serum IgA and IgM levels in piglets(P<0.05);Feed addition 5×108 CFU/kg Clostridium butyricum significantly increased the serum IL-2 content in piglets(P<0.05);Feed addition 1~10×108 CFU/kg Clostridium butyricum significantly reduces serum IL-1 in piglets β Content(P<0.05);Feed addition 5×108 CFU/kg Clostridium butyricum significantly increased the serum IL-10 content in piglets(P<0.05);In addition,Clostridium butyricum has effects on serum IgG,IL-6,and TNF in piglets-α The content had no significant effect(P>0.05).In summary,under the conditions of this experiment,5×The addition of 108 CFU/kg Clostridium butyricum has the best growth promoting effect.