To master the influence of lentinan on growth performance,immune function and immune effect of avian influenza vaccine in laying hens,a total of 120 1-day-old non-immune Roman grey chickens were randomly divided into 4 groups.The control group was not supplemented with lentinan,and the experimental groups Ⅰ,Ⅱ and Ⅲ were supplemented with 50,100 and 200 mg/kg lentinan,respectively.The experiment lasted for 77 days.The results showed that the average daily gain of Ⅰ,Ⅱ and Ⅲwas significantly higher than that of control group(P＜0.05),the ratio of feed to gain was significantly lower than that of control group(P＜0.05),and group Ⅲ was the best.Serum levels of IL-2 and IFN-γ in group Ⅲ were significantly higher than those in control group(P＜0.05).After immunization with avian influenza vaccine,the H5 antibody level of laying hens in groups Ⅰ,Ⅱ and Ⅲ was significantly higher than that in control group(P＜0.05),and group Ⅲ was the highest.The results showed that lentinan could improve the growth performance,immune function and immune effect of avian influenza vaccine of laying hens.The optimal amount of lentinan added in this experiment was 200 mg/kg.
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