Ultrasound-assisted extraction of ginseng leaf polysaccharide and optimization by central complex design
In order to establish a green and efficient process for isolating ginseng leaf polysaccharide,in this paper,water based-ultrasonic assisted extraction was used to isolate ginseng leaf polysaccharide.Central complex design(CCD)in response surface methodology was used to optimize the polysaccharide extraction yield.The polysaccharide was isolated by ethanol precipitation,and the content of polysaccharide and galacturonic acid was quantified in the extract.The results showed that the optimized process of CCD was liquid to material ratio of 21.28 mL/g,ultrasound time of 58.33 min and ultrasonic power of 456.67 W,with the predicted yield of ginseng leaf polysaccharide being 2.07％.Under the modified process,i.e.,the liquid to material ratio 21 mL/g,ultrasonic time 60 min and ultrasonic power 450 W,the extraction yield of ginseng leave polysaccharide was 1.95％;the polysaccharide content was 20.03％and galacturonic acid content was 13.79％.This process was efficient and energy saving,and could be used as a method for efficient isolation of ginseng leaf polysaccharide.
ginseng leavescentral complex designultrasound-assistedextraction and separationpolysaccharides
万方数据
维普
