首页|超声法与闪式提取法提取刺五加中刺五加苷B的工艺对比研究

超声法与闪式提取法提取刺五加中刺五加苷B的工艺对比研究

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本试验采用正交试验优化超声法和闪式提取法提取刺五加中刺五加苷B的提取工艺,并对两种提取方法的最佳工艺进行对比研究.以刺五加苷B的提取率为评价指标,设计L9(34)超声法和闪式提取法的正交试验,以高效液相色谱法测定刺五加苷B的含量.结果显示:超声法提取率高于闪式提取法,最佳提取工艺为提取溶剂30％乙醇,超声功率200 W,提取时间0.75 h,料液比1∶5(g/mL).本研究可为刺五加在饲料领域的开发和利用提供参考.
Comparative study on the extraction of eleutheroside B from Acanthopanacis senticosi Radix Et Rhizoma Seu Caulis by ultrasonic method and flash extraction method
To optimize the extraction process of eleutheroside B from Acanthopanacis senticosi Radix Et Rhizoma Seu Caulis by ultrasound and flash extraction based on orthogonal experiments,and the extraction effects of the two best processes were compared.The extraction rate of eleutheroside B was used as an evaluation indicator and the orthogonal test of ultrasonic method and flash extraction method using L9(34)was designed.The content of eleutheroside B was determined by high performance liquid chromatography method.The results showed that the extraction rate of ultrasound method was significantly higher than that of flash extraction method,the optimum extraction process was as follows:extraction solvent of 30％ethanol,extracting time of 0.75 h,ultrasonic power of 200 W,material-liquid ratio of 1∶5(g/mL).This study could provide references for the development and utilization of Acanthopanacis senticosi Radix Et Rhizoma Seu Caulis in the field of feed.

ultrasonic extractionflash extractionAcanthopanacis senticosi Radix Et Rhizoma Seu Cauliseleutheroside Bhigh performance liquid chromatography

关皎、丁思宇、季玲玲、胡子君、王军民、朱鹤云、韩丽琴

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吉林医药学院药学院,吉林吉林 132013

吉林医药学院附属医院,吉林吉林 132013

超声法 闪式提取法 刺五加 刺五加苷B 高效液相色谱法

国家自然科学基金吉林省教育厅科技项目(十三五)吉林省大学生创新创业训练计划

81703683JJKH20200466KJ202113706074

2024

10.15906/j.cnki.cn11-2975/s.2023060054-12

中国饲料
中国饲料工业协会

中国饲料

北大核心
影响因子：0.577
ISSN：1004-3314
年,卷(期)：2024.(11)
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