Effects of different extracts of Astragalus membranaceus on growth performance,serum cytokines and antioxidant indices of lambs
To study the effects of Astragalus extracted by different extraction methods on growth performance,serum cytokines and antioxidant indexes of Mongolian lambs,sixty healthy Mongolian lambs with similar body weight(22.64±1.21)kg,body size and month age were randomly selected and divided into control group,Astragalus decoction group,Astragalus ultrasonic solution group and fermented Astragalus extract group with 3 replicates per group and 5 lambs per replicate.The pre-test lasted for 10 days and the formal test lasted for 60 days.On the 60th day of the formal test,blood samples were collected intravenously,serum was separated and relevant indexes were determined.The results showed as follows:(1)Compared with the control group,the final body weight of groups Ⅰ and Ⅱ had no significant difference(P＞0.05),and the final body weight of group Ⅲ was increased by 4.5,3.71 kg and 2.46 kg compared with that of control group,groups Ⅰand Ⅱ(P＜0.05).The average daily gain in groups Ⅰ,Ⅱ and Ⅲ was 9.4,11.43 kg and 27.56 kg higher than that in control group,respectively(P＜0.05).The ratio of feed to gain in groups Ⅰ,Ⅱ and Ⅲ was decreased by 0.57,0.95 and 1.68 compared with the control group(P＜0.05).(2)Compared with the control group,the levels of interleukin-6(IL-6),interleukin-10(IL-10),interleukin-12(IL-12),tumor necrosis factor(TNF-a)and interferon-γ(IFN-γ)in group Ⅰ were reduced by 1.1,1.53,0.87,1.49 pg/mL and 1.07 pg/mL(P＜0.05).Compared with group Ⅱ,the contents of IL-10,TNF-γ and IFN-γ in group Ⅲ were reduced by 0.78,1.14 pg/mL and 0.45 pg/mL,respectively(P＜0.05).The contents of IL-6,IL-10 and IL-12 in group Ⅱ were decreased by 0.5,0.67 pg/mL and 0.72 pg/mL compared with group Ⅰ(P＜0.05).(3)Compared with the control group,the contents of glutathione peroxidase(GSH-Px),total antioxidant capacity(T-AOC)and superoxide dismutase(SOD)in group Ⅲ were increased by 2.13,2.81 U/mL and 1.79 U/mL,respectively(P＜0.05).Compared with group Ⅱ,the contents of GSH-Px,T-AOC and SOD in group Ⅲ were increased by 1.17,1.54 U/mL and 0.9 U/mL,respectively(P＜0.05).The content of catalase(CAT)in groupⅢ was increased by 2.87,2.84 U/mL and 2.37 U/mL,respectively,compared with that in groups Ⅰ and Ⅱ(P＜0.05).Serum malondialdehyde(MDA)content in group Ⅲ was decreased by 2.86,2.54 nmol/mL and 1.93 nmol/mL compared with control group,group Ⅰ and group Ⅱ,respectively(P＜0.05).In conclusion,different extraction methods of Astragalus had different effects on growth performance,serum cytokines and antioxidant indexes of lambs,and the effects of fermented Astragalus water extract group were more obvious.
Astragalusextraction methodgrowth performancecytokinesantioxidant index
万方数据
维普
