Effects of nano-composites of copper and carbon on growth performance,antioxidant capacity and intestinal mucosal barrier of yellow-feathered broilers from 1～21 days of age
The aim of this study was to investigate the effects of nano-composites of copper and carbon(NCCC)on growth performance,antioxidant capacity and intestinal barrier function of yellow-feathered broilers from 1～21 days of age,and to seek the minimum dosage of NCCC that could achieve the maximum benefit without obvious negative effects.A total of 240 one-day-old male yellow-feathered chicks were randomly divided into 4 treatment groups with 5 replicates per group and 12 broilers per replicate.The control group was fed with corn-soybean meal basal diet,and the other 3 groups were fed the basal diet supplemented with 50,100,200 mg/kg NCCC,respectively.The dosage of copper in control group and 50 mg/kg NCCC group met maximum dose limitation,while other 2 groups exceeded.The experiment lasted for 21 days.The results showed that compared with the control group,the growth performance and immune organ index of yellow-feathered broilers were not significantly affected by different levels of NCCC(P＞0.05).In terms of antioxidant function,compared with the control group,the 50,200 mg/kg NCCC groups showed an increase of 6.14％and 8.57％in the ratio of reduced glutathione(GSH)to oxidized glutathione(GSSG)in jejunum mucosa of broilers,respectively(P＜0.05).The catalase(CAT)content increased by 28.38％and 24.92％,respectively(P＜0.05).However,this also led to an increase in malondialdehyde(MDA)content by 19.27％and 13.39％,respectively(P＜0.05).As for intestinal barrier function,the addition of 50,200 mg/kg NCCC resulted in a notable increase of 32.04％and 29.80％in the secretory immunoglobulin A(sIgA)content in the jejunum mucosa of broilers compared with the control group,respectively(P＜0.05).The level of[3-defensin9(β-DF9)were also remarkably raised by 38.38％and 25.41％,respectively(P＜0.05).However,the 50,100,200 mg/kg NCCC treatment respectively inhibited the expression of CLDN-1,CLDN-3 and CLDN-1 by 28.00％,10％and 52％,respectively(P＜0.05).In summary,the addition of NCCC to the diet of yellow-feathered broilers from 1 to 21 days of age had no obvious effect on their growth performance and immune organ development,and had both advantages and disadvantages on antioxidant function and intestinal barrier function.Therefore,NCCC should be carefully considered when applied to broiler production.
yellow-feathered broilersnano-composites of copper and carbongrowth performanceintestinal barrierantioxidant capacity
万方数据
维普
