Analysis of fatty liver phenotype in juvenile largemouth bronze gudgeon(Coreius guichenoti)based on transcriptome sequencing
In order to investigate the cause of fatty liver in juvenile largemouth bronze gudgeon(Coreius guichenoti),RNA-Seq technology was conducted on the phenotypes of no abnormality liver and fatty liver.A total of 50665 Unigene was obtained by transcriptome splicing and assembly.The length of Unigene was mainly 300～1000 bp,accounting for 65.42％of the total Unigene.In comparison with Nr,GO,KEGG,Pfam,COG and Swiss-Prot databases,2438 differentially expressed genes were identified,of which 1195 were upregulated and 1243 were downregulated.KEGG metabolic pathway analysis revealed that fatty liver formation was closely linked to lipid metabolism,in particular the PPAR signaling pathway,glycerolipid metabolism,fatty acid biosynthesis,and steroid biosynthesis pathways.Further analysis of differentially expressed genes revealed that lipid synthesis genes,such as GPAT3,LPIN1,PLPP1,FASN,ACC1 and ACPM were significantly upregulated,while lipolysis related genes,such as PNPLA2,MGLL,LPL,DGKB and ACSL were significantly downregulated.Meanwhile,enzyme activities of ACC and FASN in the fatty liver group were significantly increased,while HSL and CPT1 were significantly decreased.Therefore,fatty liver accumulation was mainly caused by increased lipid synthesis and decreased lipolysis,resulting in fatty liver.To a certain extent,the results provided a theoretical basis for future study on metabolic regulation and liver disease in juvenile largemouth bronze gudgeon.
万方数据
维普
