Study on protease production from crab shell meal fermented by Bacillus subtilis
This experiment consists of crab shell powder and sucrose as a crab shell powder medium.The optimal medium composition was determined by measuring the protease activity in the fermentation broth,and the crab shell powder medium was optimized using single factor text and response surface optimization methods.Make Bacillus subtilis ferment crab shell powder to produce protease,improve the recovery rate of waste crab shell,reduce environmental pollution.The experimental results showed that when the concentration of crab shell powder was 5.12％,the inoculation amount of Bacillus subtilis was 3.02％,the concentration of sucrose was 11％,and the initial pH was 7.07,the enzyme activity of Bacillus subtilis fermented crab shell powder was the largest.The value was 17.4220 U/mL,which was a certain increase compared with the highest protease activity of 14.2290 U/mL in the single factor text.
NETL
NSTL
万方数据
