Analysis of fungal structural diversity in feces of boars and sows at different physiological phases
The aim of the study was to investigate the structural diversity of the fungi in feces of breeding boars,pregnant sows and lactating sows,so as to lay a theoretical foundation for the research of intestinal fungal function in breeding pigs and the preparation of pig feed.Fecal samples of 18 Duroc boars and 18 mature Landrace sows mated to boars during pregnant and lactating were collected for fungal ITS2 sequencing.The results showed as follows:(1)Twelve core fungal genera were shared among breeding boars,pregnant sows and lactating sows,mainly including Aspergillus,Geotrichum and Pichia,which accounted for 47.73％of the total sequences.(2)Analysis of α-diversity indicated that the Simpson index of boars was significantly higher than that of pregnant sows(P＜0.05),and the number of AS Vs,Shannon index and Chao1 index of pregnant sows were significantly higher than that of lactating sows(P＜0.05).The principal coordinate analysis showed different clusters of fecal fungal flora in breeding boars,pregnant sows and lactating sows(P=0.001).(3)The abundance of Chytridiomycota in the feces of boars was significantly higher(P＜0.05)than that of sows and the abundance of Basidiomycota was significantly lower(P＜0.05)than that of the sows.The relative abundance of Ascomycota was higher in the feces of sows during pregnancy than during lactation(P＞0.05),whereas the relative abundance of Basidiomycota was lower during lactation(P＜0.05).At the genus level,the abundance of Kazachstania,Tahromyces and Piromyces was significantly higher in boars than that in sows(P＜0.05),while the abundance of Wallemia and Candida was significantly lower in boars as compared to sows(P＜0.05).For sows at different physiological stages,the abundance of Aspergillus and Clavispora in the feces of pregnant sows was significantly higher than that of lactating sows(P＜0.05),whereas the abundance of Pichia,Candida and Penicillium was significantly lower than that in lactating sows(P＜0.05).In conclusion,the structure and diversity of fecal fungi were significantly different in boars and sows at different physiological stages,which laid a theoretical basis for the functional study of intestinal fungi in boars and sows and the research and development of feed for breeding pigs.
NETL
NSTL
万方数据
