This experiment was conducted to investigate the effects of probiotics in diet on growth,serum and rumen fermentation performance of Simmental calves.Eighteen 70-day-old Simmental calves with about same body weight were used.In the control group,calves were fed a basal diet.In the experimental groups,calves were fed the basal diet supplemented with 1.5 g and 3 g of probiotics,respectively.The pre-trial lasted for 7 d and the experimental lasted for 60 d.The results showed that:(1)The average daily gain(ADG)of group Ⅱ was significantly higher than control group by 18.31％(P＜0.05),but differences were not found in dry matter intake and feed efficiency among all groups(P＞0.05).(2)There were no significant differences in blood biochemical indices of alanine aminotransferase,aspartate aminotransferase,total protein,albumin,cholesterol and triglyceride(P＞0.05).The IgG and IL-6 of group Ⅱ was significantly higher than control group by 26.08％and 20.17％respectively(P＜0.05).Other immune indexes including immunoglobulin A,immunoglobulin M,interleukin-4 and interleukin-10 were not significantly different among all groups(P＞0.05).(3)Compared with control group,rumen ammonia nitrogen and butyric acid contents in trial group Ⅱwere significantly increased by 49.85％and 45.17％respectively,while acetic acid content was significantly decreased by 34.24％(P＜0.05).Other rumen fermentation parameters pH,propionic acid and isobutyric acid were not significantly different among groups(P＞0.05).In conclusion,supplementation of probiotics improved calves growth performance,enhanced immune function and improved rumen fermentation function of Simmental calves.In this experiment,it was more suitable to add 3 g/(head·d)compound probiotics.
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