Effects of fermented sweet potato residue feed on growth performance,intestinal morphology and non-specific immunity of carp
This study aimed to investigate the effects of fermented sweet potato pomace feed on growth performance,intestinal morphology and non-specific immunity of carp.In this experiment,after fermentation of sweet potato pomace with probiotics,the feeds were prepared according to the additive ratios of 0％,10％,20％and 30％(i.e.,control group,10％-added group,20％-added group and 30％-added group),and fed to the carp with initial body weights of(408.36±26.12)g in groups of 3 replicates of 10 carp each,and the experimental period was 42 d.Growth performance,serum antioxidant indexes and non-specific immunity indexes were measured,and sections were taken to observe immunity and immunity.The growth performance,serum antioxidant indexes and non-specific immunity indexes of the test fish were measured,and the structural morphology of the immune organs and intestines were observed in slices.The results showed that:(1)Compared with the control group,the specific growth rate of carp in the 10％and 20％additive groups increased significantly by 37.45％and 40.17％(P＜0.05).(2)The length of intestinal villi and length of intestinal villi/crypt depth(V/C)and the thickness of muscle layer were significantly increased(P＜0.01)in carp in the 10％-added,20％-added and 30％-added groups compared with the control group.(3)Compared with the control group,the spleen glutathione peroxidase(GSH-Px)content of carp in the 20％-added group was significantly increased by 27.99％(P＜0.05),the serum malondialdehyde content of carp in the 30％-added group was significantly decreased by 29.55％(P＜0.05),and the level of lysozyme(LZM)in the spleen of carp in the 10％-added and 30％-added groups was significantly decreased(P＜0.05).(4)Compared with the control group,the levels of spleen IFN-γ in the 20％-added groupwas significantly reduced by 25.84％(P＜0.05),and the levels of renal IL-1β in the 30％-added group were significantly increased by 27.84％(P＜0.05).The above results inducated that fermented sweet potato pomace could improve the intestinal morphology and structure of carp,promote the digestion and absorption of nutrients and enhance the non-specific immunity,which was conducive to the growth of carp,of which 20％of fermented sweet potato pomace was the most effective.
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