超高效液相色谱-串联质谱法测定混合型饲料添加剂中土霉素和金霉素
Study on determination of oxytetracycline and chlortetracycline in mixed feed additives by UPLC-MS/MS
胡晓颖 1孙凯 2朱娜 2张玮3
作者信息
- 1. 山东省畜牧总站,山东济南 250100
- 2. 青岛市华测检测技术有限公司,山东青岛 266100
- 3. 山东省畜产品质量安全中心,山东济南 250100
- 折叠
摘要
本.研究旨在采用超高效液相色谱-串联质谱法优化检测混合型饲料添加剂中土霉素和金霉素残留量.试样经Na2EDTA-Mcllvaine缓冲溶液提取,HLB和MCX固相萃取柱净化,甲酸铵溶液洗脱后上机测定.结果显示:该方法在2～200 μg/L内具有良好的线性关系,定量限为20 μg/kg;土霉素的空白样品平均加标回收率为86.8％～95.5％,精密度(RSD)≤4.5％;金霉素的空白样品平均加标回收率为88.1％～94.6％,RSD≤4.1％.综上,该方法适用于混合型饲料添加剂中土霉素和金霉素残留量的定性与定量检测.
Abstract
There residues of oxytetracycline and chlortetracycline in mixed feed additives were optimized by UPLC-MS/MS method.The sample was extracted by Na2EDTA-Mcllvaine,purified by solid-phase extraction,eluted with ammonium formate,and then determined on machine.The results showed that oxytetracycline and chlortetracycline detected by this method had a good linear relationship between 2～200 µg/L.The limit of quantity of the method was 20 μg/kg.The average spiked recovery of the blank sample of oxytetracycline was 86.8％～95.5％,and RSD was≤4.5％.The average spiked recovery of chlortetracycline blank sample was 88.1％～94.6％,and RSD was≤4.1％.The method had good repeatability and could be applied to detection of oxytetracycline and chlortetracycline residues in mixed feed additives.
关键词
土霉素/金霉素/混合型饲料添加剂/超高效液相色谱-串联质谱Key words
oxytetracycline/chlortetracycline/mixed feed additives/UPLC-MS/MS引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据