Study on determination of oxytetracycline and chlortetracycline in mixed feed additives by UPLC-MS/MS
There residues of oxytetracycline and chlortetracycline in mixed feed additives were optimized by UPLC-MS/MS method.The sample was extracted by Na2EDTA-Mcllvaine,purified by solid-phase extraction,eluted with ammonium formate,and then determined on machine.The results showed that oxytetracycline and chlortetracycline detected by this method had a good linear relationship between 2～200 µg/L.The limit of quantity of the method was 20 μg/kg.The average spiked recovery of the blank sample of oxytetracycline was 86.8％～95.5％,and RSD was≤4.5％.The average spiked recovery of chlortetracycline blank sample was 88.1％～94.6％,and RSD was≤4.1％.The method had good repeatability and could be applied to detection of oxytetracycline and chlortetracycline residues in mixed feed additives.
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