Rapid determination of vitaminB1and vitamin B2 in multivitamin by acid extraction
This study ained to establish a rapid acid extraction method for the determination of vitamin B1 and vitamin B2 in multivitamin.The sample was pretreated by hydrochloric acid solution to hydrolyze at 70 ℃ for 10 minutes.The separation and detection of vitamin B1 and vitamin B2 could be realized under the detection wavelength of 260 nm of high performance liquid chromatography and diode array detector.The results showed that the linearity of the two components was good in the concentration 0.1～50.0 μg/mL.The detection limit was 0.1 μg/kg,and quantitative limit was 0.3 μg/kg.The recovery rate was 82.3％～95.7％,and the relative standard deviation was 3.2％～5.9％(n=5).This method had high accuracy and sensitivity,reducing the tedious steps of determining the two target substances separately,and could meet the needs of rapid simultaneous determination of vitamin B1 and vitamin B2 in multivitamin.
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