摘要
本研究旨在建立一种酸提取快速测定复合维生素中维生素B,、维生素B2的方法.样品前处理采用盐酸溶液在70℃提取10 min后,在高效液相色谱仪联合二极管阵列检测器260 nm的检测波长下,可以实现维生素B,、维生素B2的分离和检测.结果表明:两种组分在浓度0.1～50.0 μg/mL内线性关系良好,检出限均为0.1 μg/kg,定量限均为0.3 μg/kg,加标回收率达到82.3％～95.7％,相对标准偏差为3.2％～5.9％(n=5).本方法准确度和灵敏度均较高,可减少两种目标物分别测定的繁琐步骤,可以满足复合维生素中维生素B,、维生素B2同时快速测定需求.
Abstract
This study ained to establish a rapid acid extraction method for the determination of vitamin B1 and vitamin B2 in multivitamin.The sample was pretreated by hydrochloric acid solution to hydrolyze at 70 ℃ for 10 minutes.The separation and detection of vitamin B1 and vitamin B2 could be realized under the detection wavelength of 260 nm of high performance liquid chromatography and diode array detector.The results showed that the linearity of the two components was good in the concentration 0.1～50.0 μg/mL.The detection limit was 0.1 μg/kg,and quantitative limit was 0.3 μg/kg.The recovery rate was 82.3％～95.7％,and the relative standard deviation was 3.2％～5.9％(n=5).This method had high accuracy and sensitivity,reducing the tedious steps of determining the two target substances separately,and could meet the needs of rapid simultaneous determination of vitamin B1 and vitamin B2 in multivitamin.
基金项目
江苏省市场监管局科技计划项目(苏市监科信[2022]87号)
国家科技期刊平台
NSTL
万方数据