Research on preparation of protein feed from soy sauce distiller's grains by mixed solid-state fermentation
Using Bacillus subtilis,Lactobacillus plantarum 2-41 and Saccharomyces cerevisiae SY as test strains,Maotai town distiller's grains and wheat bran provided by a Maotai town soy vinegar factory as raw materials,the solid medium of liquor grains was optimized by adding wheat bran,and then on the basis of four single factor experiments:inoculation amount,initial pH,fermentation temperature and fermentation time.Taking the crude protein content of fermented feed as the index,the technological conditions of solid-state fermentation of mixed bacteria were optimized by orthogonal test.The results showed that the optimal addition of wheat bran in the fermentation medium was 20％,that is,the total loading of the fermentation medium was 50 g,including 40 g Maotai-flavor distiller's grains and 10 g wheat bran.The optimal technological conditions of solid-state fermentation with mixed bacteria were as follows:fermentation temperature 34 ℃,fermentation time 4 days,inoculum 13％,initial pH 4.8,and three parallel experiments were carried out.The crude protein content was(27.54±0.48)％.Finally,the sensory score of the fermented feed was 16,according to the evaluation criteria,the score was located in the excellent range of 16～20,and the score reached the excellent(first-class)fermentation feed,which could be normally used for animal feeding.
mixed fermentationsoy sauce distiller's grainsprotein feedorthogonal test
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