摘要
为研究不同水平糖蜜和复合菌酶制剂对鲜食玉米秸秆青贮过程中pH和主要微生物数量变化规律的影响,采用完全随机分组设计,共设置5个处理组:对照组(CK)、A组(2％糖蜜)、B组(复合菌酶制剂)、C组(2％糖蜜+复合菌酶制剂)、D组(4％糖蜜+复合菌酶制剂),抽真空袋装青贮,每组3个重复,分别在发酵的第0、15、30、45、60、90天取样,对青贮饲料pH和乳酸菌、好氧性细菌、酵母菌、霉菌进行测定.研究结果显示:添加不同水平糖蜜和复合菌酶制剂发酵鲜食玉米秸秆过程中,pH和微生物菌落均不同程度发生了变化,各处理组pH、乳酸菌数量均显著高于对照组(P＜0.05),好氧性细菌、酵母菌数量均显著低于对照组(P＜0.05).试验表明单独添加糖蜜、复合菌酶制剂或是糖蜜与复合菌酶制剂组合添加均可以降低青贮的pH,促进乳酸的发酵,抑制有害菌的生长,从而改善秸秆的发酵品质.在本试验条件下,添加2％糖蜜+复合菌酶制剂效果最好.
Abstract
The experiment was designed to investigate the effects of different levels of molasses and complex bacterial enzyme preparations on the pH and the number of main microorganisms during the silage of fresh corn stalks.A completely randomized design was adopted,and five treatment groups were set up:control group(CK),group A(2％molasses),group B(complex bacterial enzyme preparations),group C(2％molasses+complex bacterial enzyme preparations),and group D(4％molasses+complex bacterial enzyme preparation),vacuum bag silage,3 replicates per group,samples were collected on the 0,15,30,45,60,90 d of fermentation,respectively,to determine the pH of silage,lactic acid bacteria,aerobic bacteria,yeast,mold.The results showed as follows:during the fermentation process of fresh corn stalk with different levels of molasses and complex bacterial enzyme preparations,pH and microbial colonies were changed to different degrees.The pH and lactobacillus quantity in all treatment groups were significantly higher than that in control group(P＜0.05),and the quantity of aerobic bacteria and yeast was significantly lower than that in control group(P＜0.05).The results indicated that the addition of molasses alone,compound bacterial enzyme preparation or the combination of molasses and compound bacterial enzyme preparation could reduce the pH of silage,promote lactic acid fermentation,inhibit the growth of harmful bacteria,and improve the fermentation quality of straw.Under the experimental conditions,adding 2％molasses+compound bacterial enzyme preparation had the best effect.
基金项目
2022年畜牧兽医分院分院自拟课题(ZNKT-202208)
国家肉牛牦牛产业技术体系齐齐哈尔综合试验站()
现代农业产业技术体系(CARS-37)
齐齐哈尔市科技计划创新激励项目(CNYGG-2022009)
黑龙江省现代农业产业技术协同创新推广体系建设项目(黑农厅函[2021]1492号)
齐齐哈尔市领军人才梯队人才队伍建设资金()
NETL
NSTL
万方数据