Correlation analysis between fatty acid content and expression of SCD and SREBP-1 gene in different tissue of Horqin cattle
This article aims to study the differences in fatty acid content,as well as the expression levels of SCD and SREBP-1 genes involved in fatty acid metabolism regulation in different tissues of the Horqin cattle.Gas chromatography-mass spectrometry and real-time fluorescence quantitative PCR were used to detect the fatty acid content in two different tissues of Horqin cattle,namely the perirenal fat,perienteric fat,shoulder muscle,and longissimus dorsi,as well as the expression levels of the SCD and SREBP-1 genes.The results show that:(1)In saturated fatty acids(SFA),the content of C14:0,C16:0,and C18:0 in perirenal fat is significantly higher than that in the longissimus dorsi(P＜0.05).In monounsaturated fatty acids(MUFA),the content of C16:1 and total MUFA in the longissimus dorsi is higher than in perirenal fat,but no significant difference(P＞0.05).The content of each polyunsaturated fatty acid(PUFA),total PUFA,and unsaturated fatty acids(UFA)in the longissimus dorsi are all significantly higher than in perirenal fat(P＜0.05).(2)The expression level of the SCD gene in the longissimus dorsi of Horqin cattle is higher than in perirenal fat tissue,showing a similar trend to the expression of the SREBP-1 gene,but no significant difference(P＞0.05).The expression level of the SREBP-1 gene in the longissimus dorsi of Horqin cattle is significantly higher than in perirenal fat tissue(P＜0.05).These results indicate that the nutritional value and meat quality of the longissimus dorsi of Horqin cattle are higher and more beneficial to health than perirenal fat.At the same time,the SREBP-1 gene is involved in the regulation of fatty acid deposition along with the SCD gene,co-regulating intramuscular fat metabolism,thereby influencing the composition and flavor of beef fatty acids.
horqin cattleSCDgene and SREBP-1genefatty acidrelative expression level
万方数据
维普
