首页|枯草芽孢杆菌对发酵玉米秸秆营养成分、酸化和氨基酸含量的影响

枯草芽孢杆菌对发酵玉米秸秆营养成分、酸化和氨基酸含量的影响

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文章旨在探究枯草芽孢杆菌(Bacillus subtilis,BS)对发酵玉米秸秆营养成分、酸化和氨基酸含量的影响.试验按照BS添加量(0%、0.01%、0.02%和 0.04%)分为对照组,处理 1~3 组.每组 10 个重复,发酵条件为阴凉避光处发酵 60 d.处理 1~3 组玉米秸秆干物质含量显著降低(P<0.05),粗蛋白质含量显著提高(P<0.05);处理2 和 3 组玉米秸秆粗纤维和中性洗涤纤维含量显著降低(P<0.05);此外,BS添加对玉米秸秆酸性洗涤纤维、钙和磷含量无显著影响(P>0.05).处理 2 和 3 组发酵玉米秸秆pH显著降低(P<0.05),乙酸含量显著提高(P<0.05);处理1~3组发酵玉米秸秆丙酸含量线性降低(P<0.01),处理2和3组发酵玉米秸秆乳酸含量显著提高(P<0.05),此外,各组间发酵玉米秸秆氨态氮含量无显著影响(P>0.05),同时本试验未检测到丁酸.处理 2 和 3 组发酵玉米秸秆中甘氨酸、丙氨酸、天冬氨酸、赖氨酸含量显著提高(P<0.05),处理 1~3 组发酵玉米秸秆缬氨酸、苯丙氨酸、谷氨酸、脯氨酸含量显著提高(P<0.05);处理 2 和 3 组发酵玉米秸秆中必需、非必需氨基酸以及总氨基酸含量均显著提高(P<0.05).本试验结果表明,枯草芽孢杆菌对玉米秸秆的发酵品质具有显著的正向提升作用,认为 0.04%BS添加量的发酵效果最佳.
Comparative study on acidification and degradation of rice straw feed by different ferments
This study aims to investigate the effects of Bacillus subtilis(BS)on the nutritional composition,acidification,and amino acid content of fermented corn stover.The experiment was divided into control groups and treatment groups of 1~3 according to the addition amount of BS(0%,0.01%,0.02%,and 0.04%).Each group has 10 replicates,and the fermentation conditions are to ferment in a cool and dark place for 60 days.The dry matter content of corn straw in groups 1~3 was significantly reduced(P<0.05),while the crude protein content was significantly increased(P<0.05);The content of crude fiber and neutral washing fiber in corn stover significantly decreased in groups 2 and 3(P<0.05);In addition,the addition of BS had no significant effect on the acid washing fiber,calcium,and phosphorus content of corn straw(P>0.05).The pH of fermented corn stover in groups 2 and 3 was significantly reduced(P<0.05),and the acetic acid content was significantly increased(P<0.05);The propionic acid content of fermented corn stover in groups 1~3 decreased linearly(P<0.01),while the lactate content of fermented corn stover in groups 2 and 3 significantly increased(P<0.05).In addition,there was no significant effect on the ammonia nitrogen content of fermented corn stover among groups(P>0.05),and no butyric acid was detected in this experiment.The content of glycine,alanine,aspartic acid,and lysine in the fermented corn stover of groups 2 and 3 was significantly increased(P<0.05),while the content of valine,phenylalanine,glutamic acid,and proline in the fermented corn stover of groups 1~3 was significantly increased(P<0.05);The content of essential and non essential amino acids,as well as total amino acids,in the fermented corn straw of groups 2 and 3 were significantly increased(P<0.05).The results of this experiment indicate that Bacillus subtilis has a significant positive effect on the fermentation quality of corn straw,and it is believed that the optimal fermentation effect is achieved with a 0.04%BS addition.

corn strawBacillus subtilisfermentation qualitynutrient compositionamino acid

张桂堂、任军玲

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安阳市农业技术推广站,河南安阳 455000

玉米秸秆 枯草芽孢杆菌 发酵品质 营养成分 氨基酸

2024

中国饲料
中国饲料工业协会

中国饲料

北大核心
影响因子:0.577
ISSN:1004-3314
年,卷(期):2024.(14)