首页|基于LC-MS非靶向代谢组学分析新疆南疆地区羊肉中代谢物差异

基于LC-MS非靶向代谢组学分析新疆南疆地区羊肉中代谢物差异

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选择新疆南疆地区广泛饲养的多浪羊为试验对象,饲喂含有盐酸克伦特罗、莱克多巴胺、沙丁胺醇三种β-受体激动剂的混合试剂 21 d,分别在 7、14、21 d屠宰采集羊肉样本,使用LC-MS非靶向代谢组方法分析饲喂β-受体激动剂混合试剂样本及未饲喂β-受体激动剂混合试剂样本之间的代谢差异,筛选差异代谢物及功能注释.结果显示,β-受体激动剂处理的 3 个不同时间点共同富集代谢通路有磷酸戊糖途径,泛酸盐和辅酶A生物合成,谷胱甘肽代谢,其中磷酸戊糖代谢途径极为显著.参与磷酸戊糖代谢途径的关键代谢物为 6 磷酸葡萄糖,5-磷酸-D-核酮糖,4-磷酸-D-赤藓糖.这些代谢物可以作为检测新疆南疆地区羊肉中β-受体激动剂残留的参考标志物.
The difference of metabolites in mutton from southern Xinjiang was analyzed based on LC-MS non-targeted metabolomics
Duolang sheep,which were widely reared in southern Xinjiang,were fed with a mixture of β-adrenoceptor agonists including Clenbuterol,Ractopamine,salbutamol for 21 days,mutton samples were slaughtered at 7,14 and 21 days,the LC-MS non-targeted metabolome method was used to analyze the metabolic differences between samples fed with β-agonist mixed reagents and those not fed with β-agonist mixed reagents,and to screen for differential metabolites and functional annotations.The results:β-agonist treatment co-enriched metabolic pathways at three different time points pentose phosphate pathway,Pantothenates,Coenzyme a biosynthesis,Glutathione metabolism,the pentose phosphate metabolic pathway is very significant.The key metabolites involved in pentose phosphate metabolism are 6 glucose phosphate,5-phosphate-D-ribulose,4-phosphate-D-erythritose.These metabolites can be used as reference markers to detect β-adrenoceptor agonist residues in mutton from southern Xinjiang.

differential metabolitesbeta-agonistLC-MS

邢巍婷、吕雪峰、宫平、胡波、许艳丽、胡昕、王乐

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新疆畜牧科学院畜牧业质量标准研究所,新疆乌鲁木齐 830052

新疆畜产品质量安全重点实验室,新疆乌鲁木齐 830052

差异代谢物 β-受体激动剂 LC-MS

2024

中国饲料
中国饲料工业协会

中国饲料

北大核心
影响因子:0.577
ISSN:1004-3314
年,卷(期):2024.(14)