The effect of deworming on the levels of immunoglobulin in the feces of Przewalski horses
Relying on non-invasive sampling and ELISA technology,the levels of immunoglobulins(IgG,IgA,IgM)in the feces of Przewalski's horse by anthelmintic were used as detection indicators to study the effect of deworming on the level of immunoglobulins in the feces of Przewalski's horse,and to explore the factors affecting the level of body immunoglobulins.Six species of Gasterophilus were identified from Przewalski's horse,namely G.pecorum,G.intestinalis,G.inermis,G.nigricornis,G.haemorrhoidalis and G.nasalis.After 4 days of deworming,there was no discharge of Gasterophilus larvae in the feces,and after 6 days,the reduction rate of parasitic eggs in the feces reached 99.65％.The results showed that the average levels of IgM,IgA and IgG in the feces of Przewalski's horse before deworming were(2.232±0.592)μg/g,(13.025±2.857)μg/g and(362.684±149.403)ng/g,the levels of three kinds of immunoglobulins in females were lower than those in males,but there was no significant difference between males and females and between the two experimental populations.The average levels of IgM,IgA and IgG in the feces of Przewalski's horse after deworming were(3.250±1.701)μglg,(25.580±18.525)μg/g and(308.02±309.6056)ng/g,the overall levels of IgM and IgG in females were higher than those in males,while the overall levels of IgA in males were higher than those in females.Spearman correlation analysis was conducted between the number of Gasterophilus infected with Przewalski's horse and the rate of changes in fecal immunoglobulin.It was found that there was no significant negative correlation with IgM change rate(r=-0.770,P＞0.05),no significant negative correlation with IgA change rate(r=-0.491,P＞0.05),and a significant negative correlation with IgG change rate(r=-0.721,P＜0.05).Research had confirmed that fecal immunoglobulin could serve as one of the important indicators for evaluating the body's immune system.Scientific and reasonable deworming could improve the immune capacity of Przewalski's horse to a certain extent,and timely deworming in winter was very necessary.
万方数据
维普
