Effects of dietary squalene supplementation alleviates LPS-induced growth performance,metabolic organs function and antioxidant capacity in a piglet model
The purpose of the experiment was to study the effects of supplementing dietary squalene(SQ)on the growth performance,metabolic organ,and antioxidant capacity which were stimulated by lipopolysaccharide(LPS)in a piglet model.Thirty 35-day-old castrated male commercial piglets with similar body weights were randomly divided into three groups(CON,LPS,SQL groups)with five replicates in each group and two piglets in each replicate.The CON and LPS groups were fed a basic diet,and the SQL group was fed a basal diet with 300 mg/kg SQ.The pre-feeding period was 7 days and the formal feeding period was 10 days.On the 5th and 8th day of the test,the LPS and SQL group were intraperitoneally injected with 100μg/kg BW LPS,the CON group was injected with the same amount of normal saline.During the test,the feed intake was recorded,and the diarrhea index and breathing conditions were recorded daily from the fifth day.After the test,all piglets were weighed and slaughtered,and the blood,liver,and kidney samples were collected to detect organ function,immune and antioxidant indexes.The results showed that compared with the LPS group,the final weight,average daily feed intake(ADFI),and average daily gain(ADG)of piglets in the SQL group increased by 5.56％,13.64％and 32％,respectively,with significant differences(P＜0.05).The diarrhea index and cough and asthma index decreased by 27.59％and 32.81％,respectively,with significant differences(P＜0.05),and there was no significant difference(P＞0.05)compared with the CON group.Compared with the LPS group,the liver index and kidney index of piglets in the CON group decreased by 10.33％and 12％,respectively.The liver index and kidney index of piglets in the SQL group decreased by 6.36％and 6.61％,respectively,with significant differences(P＜0.05).The levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),and bilirubin in the serum liver function indicators of piglets in the CON group decreased by 17.31％,20.15％and 48.05％compared to the LPS group,respectively.The levels of ALT,AST,and bilirubin in the serum liver function indicators of piglets in the SQL group decreased by 12.03％,11.56％and 11.69％compared with the LPS group,respectively,with significant differences(P＜0.05).The levels of urea nitrogen(BUN)in the renal function indicators of piglets in the CON group and SQL group decreased by 27.39％and 14.64％compared with the LPS group,respectively,with significant differences(P＜0.05).Compared with the LPS group,serum superoxide dismutase(SOD)and total antioxidant capacity(TAC)of the CON group increased by 29.45％and 39.5％,liver glutathione peroxidase(GSH-Px)and TAC increased by 65.94％and 66.17％,and kidney TAC increased by 43.41％,with significant differences(P＜0.05).The serum SOD and TAC of piglets in the SQL group were increased by 22.02％and 35.61％,respectively.The GSH-Px and TAC in the liver were increased by 47.85％and 50.75％,respectively.The TAC in the kidneys was increased by 31.34％,with significant differences(P＜0.05).The levels tumor necrosis factor(TNF-α),interleukin-6(IL-6)and interleukin-1β(IL-1β)in serum of CON group decreased by 26.63％,28.42％and 41.36％,respectively compared with the LPS group,with significant differences(P＜0.05).TNF-α,IL-6,and IL-1β in serum of piglets in SQL group decreased by 18.97％,25.50％and 27.51％,respectively compared with the LPS group,with significant differences(P＜0.05).Compared with the LPS group,the relative expression levels of TNF α,IL-6 and IL-1β mRNA in the liver of piglets in the CON group decreased by 49.24％,52.38％and 48.45％,respectively,and the relative expression levet of TNF-α,IL-6 and IL-1β mRNA in the kidneys decreased by 39.76％,51.46％and 37.11％,respectively,with significant differences(P＜0.05).The relative expression levels of TNF-α,IL-6 and IL-1β mRNA in the liver of piglets in SQL group decreased by 45.18％,49.52％and 41.24％,respectively,and the relative expression levels of TNF-α,IL-6 and IL-1β mRNA in the kidneys decreased by 36.75％,32.70％and 33.96％,respectively,with significant differences(P＜0.05),and the CON group was significantly lower than the SQL group(P＜0.05).In summary,adding SQ to the diet could improve the growth inhibition,metabolic organ dysfunction,and oxidative stress caused by LPS stimulation in piglets,thereby reducing the damage caused by LPS stimulation in piglets.
squalenelipopolysaccharidepigletsgrowth performancemetabolic organ functionantioxidation
万方数据
维普
