目前,持久性有机污染物带来的生态风险和环境污染日益严重,尤其是抗生素在生产过程中残留大量有毒有害的有机废弃物抗生素菌渣.黑水虻(Hermetia illucens)是处理有机废弃物的良好选择,具有抗逆性强等特点.试验以黑水虻幼虫作为载体,选取磺胺类(SAs)抗生素作为有机污染物,设置不同浓度梯度的SAs,探究黑水虻幼虫降解SAs的可行性及其体内营养成分的变化.研究发现:黑水虻幼虫抗逆性很好,幼虫的生长发育没有受到抑制,其营养成分中粗蛋白质的含量由31.18％升高到54.60％,并且对SAs的降解效率可达80.47％,比无黑水虻幼虫降解的33.56％有明显提升,最重要的是在幼虫体内未检测到SAs的存在.通过进一步对幼虫肠道微生物进行16S rRNA分析发现,Enterococcus、Bacteria_unclassified、Enterobacteriaceae_unclassified 和 Dysgonomonas 对 SAs 的胁迫响应明显.综合黑水虻幼虫的生长性能、幼虫营养成分、SAs的降解率以及肠道微生物分析结果,幼虫对SAs的降解是一种更实用、更环保的可行性方案,并且还可以增加幼虫体内的粗蛋白质含量.
Degradation of sulfonamide antibiotics by black soldier fly and analysis of their nutrient composition
At this stage,the ecological risks and environmental pollution caused by persistent organic pollutants are becoming increasingly serious.Especially,antibiotics leave a large amount of toxic and harmful organic wasteantibiotic residue in the production process.The black soldier fly(Hermetia illucens)is a good candidate for treating organic waste with characteristics such as strong resistance.The larvae of black soldier fly were used as carriers,and sulfonamide(SAs)antibiotics were selected as organic pollutants,and different concentration gradients of SAs were set to investigate the feasibility of SAs degradation by black soldier fly larvae and the changes of nutrient composition in their bodies.It was found that black soldier fly larvae were very resistant,the growth and development of larvae were not inhibited,the content of crude protein in their nutrients increased from 31.18％to 54.60％,and the degradation efficiency of SAs reached 80.47％,which was significantly higher than that of 33.56％without black soldier fly larvae,and most importantly,no SAs were detected in the larvae.Further 16S rRNA analysis of larval gut microbes revealed that Enterococcus,Bacteria_unclassified,Enterobacteriaceae_unclassified and Dysgonomonas responded significantly to the stress of SAs.Combining the growth performance of black soldier fly larvae,the larval nutrient composition of dried worms,the degradation rate of SAs and the results of gut microbial analysis,the degradation of SAs by larvae was a more practical and environmentally feasible solution,and it could also increase the crude protein content in larvae.
万方数据
维普
