The effects of lactobacillus on growth performance,diarrhea rate,serum immunity,and rumen fermentation parameters of calves
This experiment aims to investigate the effects of lactobacillus on the growth performance,diarrhea rate,serum immunity,and rumen fermentation parameters of calves.Male calves with good developmental status and similar weight after about 100 days were selected as the experimental subjects and divided into 4 groups.Each group of calves was supplemented with 0%,0.1%,0.2%,and 0.3% lactobacilli in the basic diet,with 3 replicates in each group,5 calves in each replicate,for a total of 60 calves.The pre trial period is 10 days,and the main trial period is 60 days.Result:(1)Compared with the basal diet(with a lactobacillus addition level of 0% ),when the lactobacillus addition level was 0.2%,the final weight and average daily weight gain of calves were significantly increased(P<0.05),and the feed to weight ratio was significantly reduced(P<0.05).Different levels of lactobacillus addition significantly reduced the diarrhea rate of calves(P<0.05).(2)Compared with the basal diet(with 0% lactobacillus addition level),the levels of immunoglobulin G and interleukin-4 in calf serum were significantly increased at 0.2% and 0.3% lactobacillus addition levels(P<0.05).Different levels of lactobacillus addition significantly increased the content of immunoglobulin A in calf serum(P<0.05)and significantly reduced tumor necrosis factor in calf serum-α Content(P<0.05).(3)Compared with the basal diet(with a lactobacillus addition level of 0% ),when the lactobacillus addition levels were 0.2% and 0.3%,the pH value significantly decreased(P<0.05),and the total volatile fatty acid,acetic acid content,and ethylene to propylene ratio significantly increased(P<0.05).Different levels of lactobacillus addition significantly increased the ammonia nitrogen content(P<0.05).Conclusion:Adding different levels of lactobacillus to the basic diet can improve the growth performance of calves,reduce diarrhea rate,and effectively improve serum immunity and rumen fermentation parameters.Under the conditions of this experiment,the appropriate level of lactobacillus addition is 0.2% .