Effects of α-Glyceryl monolaurate on growth performance,intestinal immunoactivity substances and intestinal inflammatory response gene expression of grass carp
Effects of α-monolaurin(α-GML)on growth performance,intestinal immune active substances and intestinal inflammatory response gene expression of grass carp.720 grass carp with an initial weight of(213.74±7.83)g were divided into four groups,which were fed experimental diets supplemented with 0,200,400 and 800 mg/kg α-GML,respectively.(Results)Compared with control group,FBW of grass carp in 400 and 800 mg/kg α-GML groups was significantly increased by 7.29% and 8.71%,respectively(P＜0.05).PWG was significantly increased by 10.77% and 10.80% (P＜0.05),respectively.SGR was significantly increased by 5.94% and 5.94% (P＜0.05),respectively.FE was significantly increased by 10.02% and 11.01%,respectively.In addition,α-GML had no significant effect on FI of grass carp(P＞0.05).The LZ activity in the foregut of grass carp supplemented with 400 and 800 mg/kg α-GML groups was significantly increased(P＜0.05);The IgM content of grass carp midgut in 200-800 mg/kg α-GML group was significantly increased(P＜0.05);In addition,the ACP activity of hind intestine of grass carp in 400 and 800 mg/kg α-GML groups was significantly increased(P＜0.05);In addition,α-GML supplementation had no significant effects on ACP activity and IgM content in foregut,LZ and ACP activities in midgut,LZ activity and IgM content in hindgut(P＞0.05).The expression levels of TNF-α and IL-6 genes in hindgut of grass carp in 200 mg/kg α-GML group were significantly decreased(P＜0.05).The expression levels of IL-1β in midgut and TNF-α and IL-6 in hindgut of grass carp in 400 mg/kg α-GML group were significantly decreased(P＜0.05).The expressions of TNF-α,TNF-α,IL-1β and IL-6 genes in foregut,midgut and hindgut of grass carp in 800 mg/kg α-GML group were significantly decreased(P＜0.05).In addition,α-GML inhibited IL-6 in foregut and midgut and IL-1β in hindgut of grass carp(P＞0.05).In summary,α-GML can promote the growth performance of grass carp by improving intestinal immunity and function.It is suggested that 400 mg/kg α-GML dose is the best.
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