The effects of fermented cottonseed meal on growth performance,protease activity,and intestinal peptide transport carriers in fattening pigs
The article aims to investigate the effects of fermented cottonseed meal on the growth performance,protease activity,and intestinal peptide transport carriers of fattening pigs.The experiment divided 200 fattening pigs weighing(36.49±1.34)kg into 4 groups,with 5 replicates in each group and 10 pigs per replicate.Each group replaced soybean meal with fermented cottonseed meal at 0%,1%,2%,and 4%,respectively.The experiment lasted for 100 days,with a pre feeding period of 10 days and a main trial period of 90 days.On the basis of no significant difference in initial weight(P＞0.05),the addition of 2% and 4% fermented cottonseed significantly reduced the FBW of fat pigs by 3.12% and 3.83% (P＜0.05),respectively;The addition of 2% and 4% fermented cottonseed significantly reduced the ADG of fattening pigs by 4.44% and 5.57% (P＜0.05);Adding 2% and 4% fermented cottonseed significantly increased the feed to weight ratio of fat pigs by 5.51% and 5.57% (P＜0.05).The addition of 2% and 4% fermented cottonseed significantly reduced the protease activity in the duodenum of fat pigs(P＜0.05);The activities of protease and trypsin in the empty stomach of fat pigs were significantly reduced(P＜0.05)when 4% fermented cottonseed was added.The expression of CAT1 and PepT1 genes in the duodenum of fat pigs with 2% and 4% fermented cottonseed groups was significantly reduced(P＜0.05);The addition of 1% to 4% fermented cottonseed significantly reduced the expression of CAT1 and PepT1 genes in the jejunum of fat pigs(P＜0.05);The addition of 1% to 4% fermented cottonseed significantly reduced the expression of CAT1 gene in the ileum of fat pigs(P＜0.05).In summary,fermented cottonseed may inhibit the growth of fattening pigs by downregulating the activity of intestinal proteases and mRNA expression of small peptide transporters,affecting the digestion and absorption of intestinal proteins and amino acids.
fermented cottonseedfattening pigsgrowth performanceproteasepeptide transport
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