Fermentation optimization and enzymatic characterization of lipase-producing antagonistic bacteria Burkholderia pyrrocinia JK-SH007
To improve the production of lipase from endophytic antagonistic bacteria Burkholderia pyrrocinia JK-SH007 and analyze its enzymatic properties,single factor test and orthogonal design method were performed to determine the optimal conditions of lipase-producing for strain JK-SH007.The impact of temperature,pH and organic solvents on lipase catalytic reaction was also investigated.The results showed that the optimum operating conditions were found as glucose 15 g/L,peptone 20 g/L,K2HPO4 2.5 g/L,peanut oil 400 μL/50mL,at pH 8.0 and 33℃ .The optimal temperature and pH were 60℃ and 9.0,respectively.The lipase was stable between temperature 50～60℃ and pH 5.0～10.0.Moreover,the lipase had solid tolerance to methanol,ethanol and ethyl acetate,especially to methanol.The lipase had a wider fitness for temperature,pH and possess favorable characteristics to meets the basic requirements of biodiesel catalyst.This work provided important clues for the exploitation and utilization of JK-SH007 in environment management and industrial production.
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