枯草芽孢杆菌HAINUP40发酵条件的优化

Optimization of fermentation conditions for Bacillus subtilis HAINUP40

丁梦莹 ¹陈鑫 ¹王世锋 ¹周永灿 ¹蔡岩¹

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作者信息

1. 海南大学,海洋生物与水产学院,海南省热带水生生物技术重点实验室,海南海口 570228
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摘要

枯草芽孢杆菌(Bacillus subtilis)作为一种有潜力的高效生物制品与水质改善剂,其活力提升的关键在于有效地利用这一有益细菌.因此,本研究选择具有优良对抗疾病源菌和蛋白质分解能力的有益菌枯草芽孢杆菌HAINUP40 开展试验.首先,在单一变量的试验中得出影响此菌株发酵后的蛋白质酶活性、抵抗力及其存活率的主要三项关键参数,即温度、起始pH和装液体积量.然后,使用Box-Behnken响应面分析方法进一步优化这些参数,最终得出最佳的发酵环境为pH 7.5、装液体积量 62.69 mL、温度 37℃.这项研究的结果有助于推动这种有益菌株的工业规模培养和实际运用.

Abstract

Bacillus subtilis is a highly effective microbial agent and water quality improver.To increase the viable count of probiotic B.subtilis fermentation,using probiotic strain Bacillus subtilis HAINUP40(screened in our previous work)as the experimental strain.Firstly determined the three major influencing factors that(temperature,initial pH,and liquid volume)affect the protease activity,antagonistic activity and viable account basing on the result of single-factor experiment.By response surface analysis,the strain's fermentation conditions were further optimized and a perfect combination of pH 7.5,liquid volume 62.69 mL,and temperature 37℃ was achieved.This study provided data support and a theoretical basis for this probiotic strain's industrial cultivation and production application.

关键词

芽孢杆菌/培养条件优化/响应面法

Key words

Bacillus/optimization of culture conditions/response surface methodology

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出版年

2024

中国饲料

中国饲料工业协会

中国饲料

北大核心

影响因子：0.577

ISSN：1004-3314

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