Effects of hydroponic barley seedlings on production performance,blood indicators,and rumen microbiota of Hu sheep
The objective of this experiment was to determine the effects of different proportions of hydroponic barley seedlings replacing corn straw and corn silage on production performance,slaughter performance,blood indicators,and rumen microbiota in Hu sheep.Fifty Hu sheep were selected and randomly divided into five groups,and each replaction with ten sheep.The control group(CK)was given a basic diet,while the experimental groups(A,B,C and D)were respectively given 5%,10%,15% and 20% hydroponic barley seedlings.The results showed that:(1)Groups C and D had significantly lower dry matter intake(DMI)compared with group CK(P<0.05),while F/G in groups B,C and D was significantly lower than those in groups CK and A(P<0.01).(2)The slaughter rate of group D was significantly higher than that of group CK(P<0.05),and the muscle redness(a*)of experimental groups was significantly higher than that of group CK(P<0.05).(3)Group D had significantly higher high-density lipoprotein(HDL)compared with group CK(P<0.05),while the creatinine(CR)of groups C and D were significantly lower than that of group CK(P<0.05).Hydroponic barley seedlings could significantly increased the glucose(GLU)content of Hu sheep(P<0.01),and significantly increased the content of immunoglobulin G and immunoglobulin M in Hu sheep with a substitution ratio of over 15%,with a significant increase in immunoglobulin A(P<0.05).(4)The relative abundance of Fibrobacterota in groups C and D significantly increased(P<0.05),while groups C and D Ruminococcidae UCG_002 and Prevotella_UCG_003 showed significant increase in relative abundance(P<0.05),with a significant decrease in the abundance of Candidatus_Saccharimonas of group A(P<0.05).In conclusion,hydroponic barley seedlings could improve production and slaughter performance,enhance the immune capacity,regulate the rumen microbiota and function of Hu sheep,and the optimal substitution ratio was 15%~20% .