Effects of combined astaxanthin and antimicrobial peptides on immune function,intestinal morphology and cecum microbial population of laying hens in the late laying period
The experiment aimed to investigate the effects of astaxanthin and antimicrobial peptides on the immune function,intestinal morphology and cecal microbial population of laying hens in the late laying period.Select 700 healthy and similar weight 56 week old Hailan brown laying hens and randomly divide them into 7 groups with 5 replicates and 20 each.E0 was the control group,fed basal diet;E1～E6 was experimental group,with each group fed separately with different levels of astaxanthin(0.5%,1.0%,1.5% )and antimicrobial peptides(300,500 mg/kg).The pre-test period was 1 week and the trial period was 8 weeks.At the end of the experiment,blood was collected to measure immune indicators,intestinal morphology and cecal microbiota.The results showed that:(1)Immune function:the IgG,IgM,IL-2,IL-10,IFN-(and IFN-(of the test group E1～E6 were significantly higher than those of the control group E0(P<0.05).(2)Intestinal morphology:in the jejunum,the villus height of test group E3 was significantly higher than that of control group E0(P<0.05),and the crypt depths and villus crypt ratios of test groups E1～E6 were significantly different compared with those of control group E0(P<0.05).In the ileum,the villus height,crypt depth and villous crypt ratio of the test group E1～E6 were significantly different compared with the control group E0(P<0.05).(3)The number of cecal microbiota:there was a significant difference between the number of Lactobacillus and E.coli in the test group E1～E6 compared with the control group E0(P<0.05).The group E3 was optimized in every test index throughout the test period.In summary,adding astaxanthin and antimicrobial peptides to the diet could enhance the immune function of laying hens,improve the morphological structure of the jejunum and ileum,and the best effect was 1.0% astaxanthin and 300 mg/kg antimicrobial peptides added in basel diet.
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