The effect of the combination of defective pears and lactic acid bacteria on the silage of Atriplex canescens
In order to better preserve the nutritional valu e of Atriplex canescens,the experiment was carried out by adding mixing of Atriplex canescens:defective pears at the ratio of 10:0(group Ⅰ),8:2(group Ⅱ),7:3(group Ⅲ),6:4(group Ⅳ)and 5:5(groupⅤ),and then adding 1.38×106 CFU/g lactic acid bacteria for mixing,with 5 replicates in each treatment group.After silage for 90 days,the nutrient composition before and after silage was determined,the quality of silage was identified and the microbial diversity was detected.The results showed that the contents of dry matter,crude protein,neutral detergent fiber and acid detergent fiber before and after silage were significantly decreased,and the contents of soluble compounds were increased(P<0.05).The contents of lactic acid and acetic acid in silage were also significantly increased(P<0.05).The contents of lactic acid in groups Ⅱ,Ⅲ,Ⅳ and Ⅴ were 1.05%,3.58%,4.39% and 4.18%,and the contents of acetic acid were 0.92%,1.39%,1.44% and 1.37%,respectively.When the contents of propionic acid,butyric acid,pH and NH3-N/TN were decreased,the silage quality in the addition group was significantly improved,the addition amount was more than 30%,the sensory quality and fermentation quality were excellent,and the comprehensive evaluation of the addition group Ⅳ was the highest,with a score of 90.5(the score of the untreated group was 46).The abundance of Firmicutes and Lactobacillus in silage in addition group increased significantly,reaching more than 95% .Decreased the abundance of Proteobacteria,Enterobacter,Leuconostoc and other miscellaneous bacteria;The abundance of Proteobacteria in the non-added group was more than 70%,and that of other miscellaneous bacteria was more than 55% .Based on the changes of chemical composition,silage quality and relative abundance of microorganisms,the silage quality could be significantly improved by adding defective pears and lactic acid bacteria above 7:3.In this study,the effects of adding defective pears on silage quality and the optimal proportion of addition were investigated,which provided the basic experimental data for future use of defective pears resources and Atriplex canescens as feed resources.
万方数据
维普
