Isolation,identification and frmentation optimization of a strain producing microbial single-cell protein(MSCP)using agricultural straw from Cangshan Mountain of Dali
To screen a strain for efficient production of microbial single-cell protein(MSCP)using agricultural straw and explore the optimal fermentation conditions for its liquid fermentation,so as to reduce the use of soybean meal protein in feed,soil samples were collected from Canglang Peak in Cangshan Mountain,Dali,and a strain S303 was obtained that could grow using straw as the carbon source.The strain was identified by morphological observation and 26S rDNA sequence analysis.The optimum straw carbon source,temperature and pH were measured to explore the MSCP yield under the optimum liquid fermentation conditions.The results showed that a strain with high efficiency in degrading straw to produce MSCP was obtained.It was identified as a strain of Candida sake and named Candida sake S303.The optimum fermentation temperature and pH of strain S303 were 25℃and 7,respectively.The optimum fermentation period was 14 days.The dry weight yield of 2%inoculum of MSCP was 1485.91 mg/L under optimal fermentation conditions.These results indicated that S303 could effectively use agricultural straw to produce MSCP,which had great application prospects in feed and food.
NETL
NSTL
万方数据
