Study on the total flavonoids extraction process and antioxidant activity of the stem bark of Acanthopanan trifoliatus from different origins
This study aimed to optimize the extraction process of total flavonoids from the stem bark of Acanthopanan trifoliatus and compare the total flavonoid content and antioxidant activity from various regions.The response surface method using ethanol reflux extraction was employed for process optimization,while the antioxidant activity of the stem bark of Acanthopanan trifoliatus was determined using DPPH and ABTS free radical scavenging assays.The results indicated that the optimal extraction conditions were as follows:extraction time of 72 minutes,ethanol volume fraction of 51%,and a liquid-to-solid ratio of 1:40(g/mL).There was a significant variation in total flavonoid content among 11 different regions,with the highest content observed in the Maoming region of Guangdong at 3.90%and the lowest in Huaihua,Hunan at 0.55%.The stem bark of Acanthopanan trifoliatus samples from different regions exhibited notable antioxidant properties,with the highest antioxidant activity observed in the Huaihua region of Hunan,displaying IC50 values of 0.5525 mg/mL for DPPH and 0.5445 mg/mL for ABTS free radical scavenging.The optimized extraction process proved to be stable and suitable for extracting total flavonoids from the stem bark of Acanthopanan trifoliatus across various regions.Furthermore,the the stem bark of Acanthopanan trifoliatus extracts from different regions displayed substantial antioxidant activity,providing a valuable foundation for resource development in this field of study.
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