酵母复合微生态制剂对鲤鱼生长性能、免疫指标及抗氧化能力的影响
Effects of yeast complex microecological preparation on growth performance,immune index and antioxidant capacity of common carp
赵倩 1王俊贤 1高绪娜 1张志焱 1徐海燕 1谷巍 1王慧 2单宝龙1
作者信息
- 1. 山东宝来利来生物工程股份有限公司 山东省动物微生态制剂重点实验室,山东泰安 271000
- 2. 山东农业大学动物科技学院,山东泰安 271018
- 折叠
摘要
本试验旨在研究饲料中添加酵母复合微生态制剂对鲤鱼生长性能、免疫指标及抗氧化能力的影响,并确定其适宜的添加量.选用初始体重为(35±0.5)g的鲤鱼 540 尾,随机分为 6 组,每组 3 个重复,每个重复 30 尾鱼,预饲 14 d.以基础日粮为CK组,试验组分别饲喂含 0.1‰、0.5‰、1‰、2‰、3‰酵母复合微生态制剂饲料,饲养 56d后采样分析.结果表明:(1)与CK组相比,饲料中添加酵母复合微生态制剂可显著提高鲤鱼的生长性能,2‰、3‰组增重率(WGR)增加了 23.01%、24.15%(P＜0.05),特定生长率(SGR)增加了 13.79%、14.37%(P＜0.05),饲料系数(FCR)降低了 18.09%、18.59%(P＜0.05).(2)与CK组相比,饲料中添加酵母复合微生态制剂可显著提高鲤鱼的免疫指标,2‰、3‰组溶菌酶(LSZ)提高了 47.9%、48.74%(P＜0.05),免疫球蛋白M(IgM)提高了 48.31%、49.44%(P＜0.05),补体C3(C3)提高了 39.61%、39.61%(P＜0.05),碱性磷酸酶(AKP)提高了 17.05%、17.44%(P＜0.05),总蛋白(TP)提高了 31.03%、30.38%(P＜0.05).(3)与CK组相比,饲料中添加酵母复合微生态制剂可显著提高鲤鱼的抗氧化能力,2‰、3‰组总抗氧化能力(T-AOC)提高了 41.69%、41.36%(P＜0.05),超氧化物歧化酶(SOD)提高了37.63%、45.71%(P＜0.05),过氧化氢酶(CAT)提高了 41.69%、41.36%(P＜0.05),丙二醛(MDA)降低了 22.71%、20.92%(P＜0.05).由此得出,饲料中添加 2‰、3‰酵母复合微生态制剂能够显著提高鲤鱼的生长性能、血清免疫指标及肝脏抗氧化能力.基于 2‰、3‰添加量组各指标差异不显著,建议酵母复合微生态制剂的最佳添加剂量为 2‰.
Abstract
The purpose of this experiment was to study the effects of yeast compound microecological preparation on growth performance,immune indexes and antioxidant capacity of carp,and to determine the appropriate dosage.A total of 540 carp with an initial body weight of(35±0.5)g were randomly divided into 6 groups with 3 replicates per group and 30 carp per replicate.The fish were pre-fed for 14 days.The basic diet was used as the CK group,and the experimental group was fed with feed containing 0.1‰,0.5‰,1‰,2‰ and 3‰ yeast complex probiotics.After 56 days of feeding,samples were collected and analyzed.The results showed that:(1)Compared with CK group,the growth performance of carp was significantly improved by adding yeast compound microecological preparation.The weight gain rate(WGR)in 2‰ and 3‰ groups was increased by 23.01%and 24.15%(P<0.05),and the specific growth rate(SGR)was increased by 13.79%and 14.37%(P<0.05).Feed ratio(FCR)was decreased by 18.09%and 18.59%(P<0.05).(2)Compared with CK group,adding yeast composite probiotics to the feed significantly improved the immune indicators of carp.The lysozyme(LSZ)of the 2‰and 3‰groups was increased by 47.9%and 48.74%(P<0.05),immunoglobulin M(IgM)was increased by 48.31%and 49.44%(P<0.05),complement C3(C3)was increased by 39.61%and 39.61%(P<0.05),and alkaline phosphatase(AKP)was increased by 17.05%and 17.44%(P<0.05).The total protein(TP)was increased by 31.03%and 30.38%(P<0.05).(3)Compared with CK group,the antioxidant capacity of carp was significantly increased by adding yeast compound microecological preparation.The total antioxidant capacity(T-AOC)in 2‰ and 3‰ groups was increased by 31.11%and 31.87%(P<0.05).Superoxide dismutase(SOD)was increased by 37.63%and 45.71%(P<0.05),catalase(CAT)was increased by 41.69%and 41.36%(P<0.05),and malondialdehyde(MDA)was decreased by 22.71%and 20.92%(P<0.05).In conclusion,the growth performance,serum immune indexes and liver antioxidant capacity of common carp could be significantly improved by adding yeast compound microecological preparations 2‰ and 3‰ in feed.Based on the fact that there was no significant difference between 2‰ and 3‰ supplementation groups,it was suggested that the optimal dosage of yeast compound microecological preparation was 2‰.
关键词
酵母复合微生态制剂/酵母培养物/酵母细胞壁/鲤鱼/生长性能/免疫指标/抗氧化能力Key words
yeast compound microecological preparation/yeast culture/yeast cell wall/carp/growth performance/immune indexes/antioxidant capacity引用本文复制引用
基金项目
山东省泰山产业领军人才项目(tscy20200114)
出版年
2024
NETL
NSTL
万方数据