Effects of maternal fecal microbiota transplantation combined with Enterococcus faecalis on the early growth and development of chicks
The purpose of this study was to establish an artificial microbiome using the mode of"fecal microbiota+probiotic"to modulate the intestinal microbiota and health of chicks.In this experiment,the maternal fecal microbiota transplantation and Enterococcus faecalis were selected to intervene the chicks,and the following effects on the early growth and development of chicks were observed.A total of 100 1 d of age Hyline brown male chicks were randomly divided into 4 groups:control group(CON group),fecal microbiota transplantation group(FMT group),Enterococcus faecalis treatment group(EF group)and the combined treatment group(CT group),with 5 replicates in each group and 5 chicks in each replicate.The chicks in the CON group and FMT group were fed the basal diet,and the chicks in the EF group and CT group were fed the basal diet supplemented with 2×109 CFU/kg Enterococcus faecalis.In the afternoon of the first 7 days,the chicks in the CON group and EF group were fed 15 mL PBS after 2 h of feed and water deprivation,and the chicks in the FMT group and CT group were fed 15 mL prepared fecal microbiota solution after 2 h of feed and water deprivation.The experiment period was 14 days.Then,growth performance,immune organ index,serum immune index,ileum morphology,ileum goblet cell number,intestinal microbial α diversity and clustering were measured.The results showed that body weight,intestinal length,ileum ratio of villous height/crypt depth,serum LZM and IL-10 levels,and goblet cells number in the CT group were significantly(P<0.05)higher than those in CON group at 14 days of age(6.44%,11.63%,30.65%,48.02%,65.07%and 44.83%,respectively).The villous height of ileum was significantly(P<0.05)higher than that in the FMT and EF groups by 10.26%and 10.14%.Body weight,tibial length,ileum villous height and ileum ratio of villous height/crypt depth in the EF group were significantly(P<0.05)higher than those in the CON group by 8.46%,3.70%,11.16%and 38.48%.The serum LZM level and ileum villous height of chicks in the FMT group were significantly(P<0.05)higher than those in the control group(31.94%and 11.04%,respectively),and the Shannon index in the FMT group was significantly(P<0.05)higher than that in the EF group.The abundance of Alistipes,Bacterroides and Odoribacter beneficial bacteria of EF group were improved,while the abundance of Prevotella and Megamonas beneficial bacteria of FMT group improved.The abundance of beneficial bacteria of Parabacteroides and Collinsella was increased in the CT group,while the abundance of harmful bacteria of Shige lla and Bamesiella was decreased in the three experimental groups.In conclusion,the results of this study indicated that fecal microbiota transplantation combined with Enterococcus faecalis promoted the early growth and development of Hyline brown chicks,enhanced immunity,renewed intestinal microbial structure,and maintained intestinal microbial balance."Fecal microbiota+probiotics"may be a potential strategy for regulating intestinal microbiota and health in poultry.
万方数据
维普
