Effects of Bifidobacteria on fecal microorganisms in Yili horse
The aim of the study was to investigate the effects of adding different gradients of Bifidobacterium to the diet on the faecal microbial diversity of Yili horses.Nineteen 2～3 year old Yili geldings with similar body condition and the same level of feeding management were selected and randomly divided into four groups,namely,test group Ⅰ(Q1,H1),test group Ⅱ(Q2,H2),test group Ⅲ(Q3,H3),and test group Ⅳ(Q4,H4),with Q being the early stage of the experiment and H being the late stage of the experiment,in which test group Ⅰ was the control group with 4 horses replicated,and the rest of the test groups were 5 replicated horses in each group.Based on the average body weight of all horses,each horse was fed 6.4 kg of concentrate supplement and 8 kg of dry forage per day,and the concentrate supplement was added twice on the same day in the morning and in the evening,and the forage was added in four times,based on which,the morning feeding of test group Ⅱ,test group Ⅲ,test group Ⅳ were based on the 0.025%,0.05%and 0.1%of the amount of the single ration of the day,and 0.8,1.6,3.2 g of Bifidobacterium were added,respectively,with a pre-feeding period of 7 days and a full-fledged period of 50 days.Fecal samples were collected from horses before and after the test,and the fecal microbial diversity of feces was detected by high-throughput sequencing of bacterial 16S Rrna genes.The results showed that:(1)2870233 optimized sequences were obtained from horse faecal samples.(2)There was no significant difference in the diversity of intestinal flora before and after feeding in the control group(P＞0.05);there was some difference between the before and after feeding in the test group Ⅱ,but it was not significant(P＞0.05);and after feeding Bifidobacterium bifidum in the test group Ⅲ,the Simpson's index was significantly higher than the pre-feeding level(P＜0.05).(3)At the gate level,the dominant intestinal flora of Yili horses were mainly Firmicutes and Bacteroidota.(4)The main dominant genera at the genus level were Streptococcus,Rikenellaceae RC9 gut group,Fibrobacter;genus level was significantly higher than that before feeding(P<0.05).At the genus level,Streptococcus accounted for the smallest proportion of the total genus level in the late phase of test group IV compared with the other groups,and Rikenellaceae RC9 gut group accounted for a higher proportion of the total genus level than the other groups.In conclusion,the addition of Bifidobacterium additives to horse diets had a certain effect on the intestinal flora of Yili horses,and improved the biodiversity of the intestinal flora of Yili horses,which was beneficial to the health of Yili horses.
万方数据
维普
