Effect of quinoa on expression of meat quality-related genes in Luhua chicken
In order to study the effects of quinoa seeds on the expression of genes related to quality and meat quality of Luhua chicken.A total of 120 healthy 49-day-old Luhua chickens with a body weight of(1476.21±101.39)g were randomly divided into 4 treatment groups with 3 replicates per treatment and 10 chickens per replicate.Chickens in the control group(CK group)were fed a basal diet supplemented with 0 quinoa seed,and the others in experimental groups(Q4,Q8 and Q12 groups)were fed experimental diets supplemented with 4%,8%and 12%quinoa seed.The experiment lasted for 75 days(pre-test period was 3 days,and experimental period was 72 days).After feeding,the meat quality and the expression of meat quality-related genes were determined.The results showed that:(1)Compared with CK group,the relative expression level of CAPN1 gene mRNA in pectoral muscle of Q4 and Q12 groups were significantly increased(P<0.05);The relative expression levels of CAST gene and LRP1 gene mRNA in breast muscle of all experimental groups were significantly decreased(P<0.05).(2)Compared with CK group,the relative expression levels of CAPN1 gene,CAST gene in Q4 group and LRP1 gene mRNA in leg muscle of Q4 and Q8 groups were significantly increased(P<0.05).(3)The expression level of LRP1 gene was significantly positively correlated with muscle shear force(P<0.05),and the expression level of CAPN1 gene was significantly negatively correlated with drip loss(P<0.05).(4)In leg muscle tissue,LRP1 gene expression level was significantly positively correlated with cooking loss and shear force(P<0.05);The expression level of CAPN1 gene was positively correlated with cooked meat rate(P<0.05).In conclusion,dietary supplementation of quinoa seeds(raw grain)had a certain effect on the expression levels of CAST,LRP1 and CAPN1 genes in muscle tissue,and LRP1,CAST and CAPN1 genes were significantly correlated with meat quality indexes related to tenderness.
万方数据
维普
