In order to study the effects of quinoa seeds on the expression of genes related to quality and meat quality of Luhua chicken.A total of 120 healthy 49-day-old Luhua chickens with a body weight of(1476.21±101.39)g were randomly divided into 4 treatment groups with 3 replicates per treatment and 10 chickens per replicate.Chickens in the control group(CK group)were fed a basal diet supplemented with 0 quinoa seed,and the others in experimental groups(Q4,Q8 and Q12 groups)were fed experimental diets supplemented with 4%,8%and 12%quinoa seed.The experiment lasted for 75 days(pre-test period was 3 days,and experimental period was 72 days).After feeding,the meat quality and the expression of meat quality-related genes were determined.The results showed that:(1)Compared with CK group,the relative expression level of CAPN1 gene mRNA in pectoral muscle of Q4 and Q12 groups were significantly increased(P<0.05);The relative expression levels of CAST gene and LRP1 gene mRNA in breast muscle of all experimental groups were significantly decreased(P<0.05).(2)Compared with CK group,the relative expression levels of CAPN1 gene,CAST gene in Q4 group and LRP1 gene mRNA in leg muscle of Q4 and Q8 groups were significantly increased(P<0.05).(3)The expression level of LRP1 gene was significantly positively correlated with muscle shear force(P<0.05),and the expression level of CAPN1 gene was significantly negatively correlated with drip loss(P<0.05).(4)In leg muscle tissue,LRP1 gene expression level was significantly positively correlated with cooking loss and shear force(P<0.05);The expression level of CAPN1 gene was positively correlated with cooked meat rate(P<0.05).In conclusion,dietary supplementation of quinoa seeds(raw grain)had a certain effect on the expression levels of CAST,LRP1 and CAPN1 genes in muscle tissue,and LRP1,CAST and CAPN1 genes were significantly correlated with meat quality indexes related to tenderness.
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