Effects of Buzhong Yiqi powder and astragalus polysaccharides on the intestinal microflora of Ussuri raccoon dogs
The aim of this study was to explore the effects of Buzhong Yiqi powder and astragalus polysaccharides on the intestinal flora of raccoon dogs.Sixty healthy Wusuli raccoon dogs of similar age and weight,30 males and 30 females,were randomly divided into 5 groups:control group,aureomycin group(group A),astragalus polysaccharide group(group B),0.5%Buzhong Yiqi powder group(group C),and 1%Buzhong Yiqi powder group(group D).The test period was 150 days.After the end of the test period,the faeces of the cecum were taken for microbial diversity analysis.The results showed that 642600 effector sequences were detected in fecal samples,and 10921 operational taxonomic units(OTUs)were detected,belonging to 20 classes,11 orders,12 families and 17 genera in 8 phyla(relative abundance>0.1);Compared with the control group,the ACE(absence based coverage estimator)index in the feces of raccoon dogs in the four experimental groups decreased by 13.9%,11.7%,9.1%,13.7%(P<0.05),respectively,while the Chao1(Chao1 richness)index decreased by 13.9%,12.6%,9.4%and 15%(P<0.05);The Simpson index of group B increased by 107.1%(P<0.05).Astragalus polysaccharides and Buzhong Yiqi powder had a trend of reducing the richness and diversity of bacterial communities at the level of five biological classification units.Adding Buzhong Yiqi powder and astragalus polysaccharide to raccoon dog diet could promote the proliferation of beneficial bacteria and inhibit the growth of potential pathogens.
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