超高效液相色谱-串联质谱测定配合饲料中10种抗病毒类药物

Determination of 10 antiviral drugs in compound feed by ultra-high performance liquid chromatography tandem mass spectrometry

张学娜 ¹范维江 ¹杜国辉 ¹张应龙 ¹焦燕¹

扫码查看

作者信息

1. 山东商业职业技术学院,山东济南 250000
折叠

摘要

实验旨在建立超高效液相色谱-串联质谱(UPLC-MS/MS)同时检测配合饲料中 10 种抗病毒类药物的分析方法.样品用 1%甲酸水、乙腈溶液提取后,经PRIME HLB固相萃取柱净化,PC HILIC色谱柱分离,以 10 mmol/L乙酸铵(含 0.2%甲酸)和乙腈为流动相进行洗脱,基质匹配标准工作曲线定量.结果显示:10 种抗病毒类药物在浓度 1.0～100 ng/mL内线性关系良好,50、100、500 μg/kg 3 水平的加标回收率为 87.5%～106.3%,相对标准偏差1.2%～10.6%,本方法检出限为 20 μg/kg,定量限为 50 μg/kg.

Abstract

A method for detecting 10 kinds of anti-viral drugs in compound feed was established by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The samples were extracted by 1%formic acid and acetonitrile solution and purified by PRIME HLB solid phase extraction column.The 10 kinds of anti-viral drugs were separated by PC HILIC column and gradiently eluted using acetonitrile and 10 mmol/L ammonium acetate solution(containing 0.2%formic acid).The quantitative analysis was conducted using the matrix matching external standard method.The results showed that the calibration curves were in good linear relationship in the range of 1.0～100 ng/mL.The recoveries of the 10 kinds of anti-viral drugs at three spiked levels(50,100,500 μg/kg)were 87.5%～106.3%,and the relative standard deviations were 1.2%～10.6%.The detection limit was 20 μg/kg and the quantitative limit was 50 μg/kg.

关键词

抗病毒药物/超高效液相色谱-串联质谱/固相萃取/配合饲料

Key words

antiviral drugs/ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)/solid phase extraction(SPE)/compound feed

引用本文复制引用

出版年

2024

中国饲料

中国饲料工业协会

中国饲料

北大核心

影响因子：0.577

ISSN：1004-3314

段落导航