首页|超高效液相色谱-串联质谱测定配合饲料中10种抗病毒类药物

超高效液相色谱-串联质谱测定配合饲料中10种抗病毒类药物

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实验旨在建立超高效液相色谱-串联质谱(UPLC-MS/MS)同时检测配合饲料中 10 种抗病毒类药物的分析方法。样品用 1%甲酸水、乙腈溶液提取后，经PRIME HLB固相萃取柱净化，PC HILIC色谱柱分离，以 10 mmol/L乙酸铵(含 0。2%甲酸)和乙腈为流动相进行洗脱，基质匹配标准工作曲线定量。结果显示:10 种抗病毒类药物在浓度 1。0～100 ng/mL内线性关系良好，50、100、500 μg/kg 3 水平的加标回收率为 87。5%～106。3%，相对标准偏差1。2%～10。6%，本方法检出限为 20 μg/kg，定量限为 50 μg/kg。
Determination of 10 antiviral drugs in compound feed by ultra-high performance liquid chromatography tandem mass spectrometry
A method for detecting 10 kinds of anti-viral drugs in compound feed was established by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The samples were extracted by 1%formic acid and acetonitrile solution and purified by PRIME HLB solid phase extraction column.The 10 kinds of anti-viral drugs were separated by PC HILIC column and gradiently eluted using acetonitrile and 10 mmol/L ammonium acetate solution(containing 0.2%formic acid).The quantitative analysis was conducted using the matrix matching external standard method.The results showed that the calibration curves were in good linear relationship in the range of 1.0～100 ng/mL.The recoveries of the 10 kinds of anti-viral drugs at three spiked levels(50,100,500 μg/kg)were 87.5%～106.3%,and the relative standard deviations were 1.2%～10.6%.The detection limit was 20 μg/kg and the quantitative limit was 50 μg/kg.

antiviral drugsultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)solid phase extraction(SPE)compound feed

张学娜、范维江、杜国辉、张应龙、焦燕

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山东商业职业技术学院,山东 济南 250000

抗病毒药物 超高效液相色谱-串联质谱 固相萃取 配合饲料

2024

10.15906/j.cnki.cn11-2975/s.2023110028-10

中国饲料
中国饲料工业协会

中国饲料

北大核心
影响因子：0.577
ISSN：1004-3314
年,卷(期)：2024.(21)