Changes of nutritional components of moldy corn and comparison of detoxification methods
The purpose of this experiment was to study the changes of nutritional components of moldy corn and compare the detoxification methods of aflatoxin B1 of moldy corn.Corn that is a feedstuff most susceptible to aflatoxin B1 pollution was selected and its water content was increased from 13%to 18%by adding distilled water.The samples were put in a 35℃ incubator for sealed culture,and the contents of crude protein,ether extract and crude ash and the changes of various nutrients compared with those before mold were detected after the surface of corn became moldy.The content of aflatoxin B1 was determined by enzyme-linked immunosorbent assay(ELISA),and then moldy corn was detoxified by 4 common detoxification methods such as activated carbon adsorption,acid degradation,high temperature degradation and ultraviolet radiation degradation,and the toxin content was determined after detoxification.The results showed as follows:compared with that before mold,the pH of feed after mold was decreased extremely significantly(P<0.01),the contents of crude protein and ether extract were decreased significantly(P<0.05),and the contents of crude ash remained basically unchanged.The detoxification effects of 4 detoxification methods from high to low were:ultraviolet radiation degradation,high temperature degradation,lactic acid degradation and activated carbon adsorption.Therefore,the contents of crude protein and ether extract were decrease after moldy;once the toxin appears,only methods could be taken to reduce its content,but it could not be completely removed.When the feed was stored,the more water content,the more unfavorable to store,and the longer the storage time,the higher the toxin content after moldy.
万方数据
维普
