Objective:This experiment aims to investigate the effects of different levels of enzyme preparation on the growth performance,apparent nutrient digestibility,and economic benefits of beef cattle.Method:Sixty healthy Simmental cattle with the same initial body weight(415.82±13.65kg)aged about 18 months were randomly divided into 4 groups with 3 replicates per group and 5 cows per replicate.Hens in the control group were fed a basal diet,and three experimental groups were fed the basal diet supplemented with 300 mg/kg,600 mg/kg and 900 mg/kg enzyme preparations,respectively.The experiment lasted for 60 days.Results:(1)Compared with control group,supplemented with 600 mg/kg enzyme preparation,the average daily gain was increased by 11.70%(P<0.05),and feed conversion rate was decreased by 9.04%(P<0.05);The average daily gain of beef cattle increased by 10.64%(P<0.05)and feed conversion rate decreased by 7.59%(P<0.05)when 900 mg/kg of enzyme preparation was added.(2)Compared with control group,the apparent digestibility of dry matter and neutral detergent fiber of beef cattle supplemented with 300 mg/kg enzyme preparation was significantly increased(P<0.05);The apparent digestibility of dry matter,crude protein and neutral detergent fiber of beef cattle was significantly increased by adding 600~900 mg/kg enzyme preparation(P<0.05).(3)The gross profit of the four groups of beef cattle was 740.33 yuan,807.15 yuan,904.55 yuan and 868.29 yuan,respectively.Conclusion:The growth performance,nutrient apparent digestibility and economic benefit of Simmental beef cattle could be improved by adding enzyme preparation to base diet.Considering the feeding effect of beef cattle comprehensively,the appropriate supplemental level of enzyme preparation under the experimental conditions is 600 mg/kg.