The effect of mulberry branch powder on the growth performance and skeletal muscle development of broiler chickens.720 1-day-old Aba Yijia(AA)broiler chickens(average weight 45.8±1.29g)were randomly divided into 4 groups,with 6 replicates in each group and 30 chickens per replicate.They were fed in two stages of 1-3 weeks and 4-6 weeks,with 0%,1.5%,3.0%,and 6.0%mulberry branch powder added to the basic feed at each stage as the experimental feed.Compared with the control group,the final weight of broiler chickens in groups Ⅱ and Ⅲ significantly increased by 2.43%and 2.20%(P<0.05),the average daily weight gain increased by 2.49%and 2.24%(P<0.05),and the feed to meat ratio significantly decreased by 3.24%and 2.85%(P<0.05).The mortality rates of broiler chickens in groups Ⅰ-Ⅲ decreased by 46.65%,39.23%,and 34.41%,respectively,compared with the control group(P<0.05);In addition,each mulberry branch powder had no significant effect on the feed intake of broiler chickens(P>0.05).In the third week,the chest and leg muscle rates of Group Ⅲ broiler chickens significantly increased(P<0.05),and in the sixth week,the muscle rates of meat chicken legs in Groups Ⅱ and Ⅲ significantly increased(P<0.05).In the third week,the expression of IGF-1R and mTOR genes in Ⅱ and Ⅲ chicken leg muscles was significantly upregulated(P<0.05),and the expression of Pax7 gene in group Ⅲ was significantly upregulated(P<0.05);In the 6th week,the expression of IGF-1R gene in Ⅱ and Ⅲ chicken leg muscles was significantly upregulated(P<0.05),and the expression of m-TOR gene in group Ⅲ was significantly upregulated(P<0.05).In addition,mulberry branch powder had no significant effect on the expression levels of MSTN,MuRF1,and MyoD genes in the leg muscles of broiler chickens at all stages(P>0.05).(Experimental conclusion)Mulberry branch powder(6%)can improve the growth performance of broiler chickens and promote leg muscle development.