The effect of sorghum diet on the growth performance,serum biochemical indicators,and gut microbiota of meat ducks.1200 healthy 1-day-old Cherry Valley male ducks were randomly divided into 4 groups,with 10 replicates in each group and 40 ducks per replicate.The control group(Ctr)and experimental groups Ⅰ,Ⅱ,and Ⅲ were fed with diets supplemented with 0%,3%,6%,and 12%sorghum,respectively,for a total of 35 days.The results showed that the final weight of experimental group Ⅲ meat ducks was significantly lower than that of the control group by 8.08%(P<0.05).The average daily weight gain of experimental group Ⅱ and Ⅲ meat ducks was significantly lower than that of the control group by 5.52%and 8.30%,respectively(P<0.05).The feed meat of experimental group Ⅲ meat ducks was significantly reduced by 7.79%compared to the control group(P<0.05);In addition,there was no significant difference in feed intake between the groups(P>0.05).The serum TP and T4 levels in groups Ⅱ and Ⅲ meat ducks were significantly reduced(P<0.05),while ALP and ALT activities were significantly increased(P<0.05).The serum AST activity in group Ⅱ meat ducks was significantly reduced(P<0.05),and the serum T-AOC activity in group Ⅲ meat ducks was significantly reduced(P<0.05);There were no significant differences in serum Alb,Ca,P,C3,C4,and MDA levels among the groups of meat ducks(P>0.05).The content of Escherichia coli and Bacillus subtilis in the cecum of meat ducks significantly increased(P<0.05),while the content of Salmonella,Lactobacillus,and Clostridium butyricum in the cecum of meat ducks did not show significant differences among the groups(P>0.05).(Experimental conclusion)Excessive(12%)addition of sorghum has a certain negative impact on the growth and development of meat ducks,and its mechanism may be related to the damaging effect of sorghum on their intestines and internal organs.