Fast determination of Benzo (a) pyrene in fried oil by high performance liquid chromatography
benzo (a)pyrene is a common high activity indirect carcinogens, benzo (a)pyrene through a food processing and other way into food, High - performance liquid chromatography ( HPLC) was used for determination of benzo (a) pyrene in fried oil. C18 as the separation column. The mobile phase of acetonitrile; water, excitation wavelength of fluorescence detector 384nm and detection wavelength 406 nm. Temperature of column 30℃ , and the injection volume of 10μl. The calibration curve is linear in the range of 1 ~40μg/L, Y = 1. 5758X +0. 05705, R2 = 0. 9998, the limit of detection was 0. Μg/kg, recoveries were more than 95% , and relative standard deviation (RSD)was no more than 2. 5%. The method has advantages of an accuracy and simple process of sample treatment, short analysis time, is suitable for qualitative and quantitative analysis of benzo (a)pyrene in fried oil, and is a good detection method of benzo (a)pyrene in fried oil.
国家科技期刊平台
NSTL
万方数据
维普
