Objective:To evaluate the inhibition activities of apigenin (AP) on mice intestinal α-glucosidase.Methods:In vitro α-glucosidase inhibitory model was established to determine the inhibition activities on α-glucosidase,maltose and sucrose by AP.Furthermore,Lineweaver-Burk mapping method was adopted to determine the type of inhibition of AP.,andThe isolated mice intestine in vitro experiment was used to understand the stability of inhibition on maltose and sucrose.Results:The IC50 of AP on α-glucosidase,maltose and sucrose were 14.475μg/mL,165.35μg/mL and 149.57μg/mL,respectively.Maltose and sucrose were inhibited by AP via reversible competitiveness,and the constant of inhibition (Ki) were 32.14μg/mL and 40μg/mL,respectively.In the isolated mice intestinal system,the inhibition of AP were steady-going during 60min.Conclusions:AP can competitively inhibit mice intestinal maltase and sucrose in mice intestinal.,tTherefore,it Can bemay be used to reduce the postprandial blood glucose in diabetes patients with diabetes mellitus.
国家科技期刊平台
NSTL
万方数据
维普
